Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 4015520..4016321 | Replicon | chromosome |
| Accession | NZ_CP079992 | ||
| Organism | Escherichia coli strain KMS2001 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | D8EBK0 |
| Locus tag | KYK85_RS19465 | Protein ID | WP_001094436.1 |
| Coordinates | 4015944..4016321 (+) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | B7MN76 |
| Locus tag | KYK85_RS19460 | Protein ID | WP_015953067.1 |
| Coordinates | 4015520..4015897 (+) | Length | 126 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KYK85_RS19425 (4011432) | 4011432..4012112 | + | 681 | WP_001282927.1 | WYL domain-containing protein | - |
| KYK85_RS19430 (4012260) | 4012260..4012937 | + | 678 | WP_001097312.1 | hypothetical protein | - |
| KYK85_RS19435 (4012943) | 4012943..4013176 | + | 234 | WP_001278287.1 | DUF905 family protein | - |
| KYK85_RS19440 (4013266) | 4013266..4014084 | + | 819 | WP_001175142.1 | DUF932 domain-containing protein | - |
| KYK85_RS19445 (4014175) | 4014175..4014660 | + | 486 | WP_000860054.1 | antirestriction protein | - |
| KYK85_RS19450 (4014675) | 4014675..4015151 | + | 477 | WP_001186756.1 | RadC family protein | - |
| KYK85_RS19455 (4015220) | 4015220..4015441 | + | 222 | WP_000692312.1 | DUF987 domain-containing protein | - |
| KYK85_RS19460 (4015520) | 4015520..4015897 | + | 378 | WP_015953067.1 | type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| KYK85_RS19465 (4015944) | 4015944..4016321 | + | 378 | WP_001094436.1 | TA system toxin CbtA family protein | Toxin |
| KYK85_RS19470 (4016318) | 4016318..4016806 | + | 489 | WP_000761714.1 | DUF5983 family protein | - |
| KYK85_RS19475 (4016818) | 4016818..4017015 | + | 198 | WP_000839254.1 | DUF957 domain-containing protein | - |
| KYK85_RS19480 (4017100) | 4017100..4017945 | + | 846 | WP_001280513.1 | DUF4942 domain-containing protein | - |
| KYK85_RS19485 (4018016) | 4018016..4019551 | + | 1536 | WP_000492897.1 | EAL domain-containing protein | - |
| KYK85_RS19490 (4019948) | 4019948..4020118 | + | 171 | Protein_3827 | IS110 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | papG / papF / papE / papK / papJ / papD / papC / papH / papA / papB / papI / kpsF / kpsE / kpsE / kpsD / kpsU / kpsC / kpsS / neuE / neuC / neuA / neuB / neuD / kpsT / kpsM / gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspD / gspC / rfaE | 3964796..4158456 | 193660 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 13958.84 Da Isoelectric Point: 6.8603
>T210510 WP_001094436.1 NZ_CP079992:4015944-4016321 [Escherichia coli]
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ
MNTLPDTHVREASGCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKQ
Download Length: 378 bp
>T210510 NZ_CP104533:3657772-3657990 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA
Antitoxin
Download Length: 126 a.a. Molecular weight: 13766.60 Da Isoelectric Point: 6.2021
>AT210510 WP_015953067.1 NZ_CP079992:4015520-4015897 [Escherichia coli]
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV
VSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTITLYAKGLTCEADTLGSCGYVYLAVYPTPAAPAITV
Download Length: 378 bp
>AT210510 NZ_CP104533:3657372-3657746 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0E2L1N0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A5Z3CIS0 |