Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3741141..3741724 Replicon chromosome
Accession NZ_CP079992
Organism Escherichia coli strain KMS2001

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag KYK85_RS18195 Protein ID WP_000254750.1
Coordinates 3741141..3741476 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KYK85_RS18200 Protein ID WP_000581937.1
Coordinates 3741476..3741724 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK85_RS18180 (3737027) 3737027..3738325 - 1299 WP_000036723.1 phosphopyruvate hydratase -
KYK85_RS18185 (3738413) 3738413..3740050 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KYK85_RS18190 (3740278) 3740278..3741069 - 792 WP_001071651.1 nucleoside triphosphate pyrophosphohydrolase -
KYK85_RS18195 (3741141) 3741141..3741476 - 336 WP_000254750.1 endoribonuclease MazF Toxin
KYK85_RS18200 (3741476) 3741476..3741724 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KYK85_RS18205 (3741802) 3741802..3744036 - 2235 WP_000226815.1 GTP pyrophosphokinase -
KYK85_RS18210 (3744084) 3744084..3745385 - 1302 WP_000046816.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T210508 WP_000254750.1 NZ_CP079992:c3741476-3741141 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T210508 NZ_CP104533:2813977-2814084 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT210508 WP_000581937.1 NZ_CP079992:c3741724-3741476 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT210508 NZ_CP104533:c2813924-2813863 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References