210507

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 3605819..3606486 Replicon chromosome
Accession NZ_CP079992
Organism Escherichia coli strain KMS2001

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag KYK85_RS17535 Protein ID WP_001094400.1
Coordinates 3606157..3606486 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag KYK85_RS17530 Protein ID WP_000072690.1
Coordinates 3605819..3606136 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK85_RS17500 (3600871) 3600871..3601880 - 1010 Protein_3438 arsenic transporter -
KYK85_RS17505 (3602022) 3602022..3603725 + 1704 WP_000896263.1 protein YfjW -
KYK85_RS17510 (3604336) 3604336..3604520 + 185 Protein_3440 DUF905 family protein -
KYK85_RS17515 (3604623) 3604623..3605081 + 459 WP_000211841.1 antirestriction protein -
KYK85_RS17520 (3605090) 3605090..3605572 + 483 WP_240275544.1 RadC family protein -
KYK85_RS17525 (3605581) 3605581..3605781 + 201 WP_001395452.1 DUF987 domain-containing protein -
KYK85_RS17530 (3605819) 3605819..3606136 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KYK85_RS17535 (3606157) 3606157..3606486 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
KYK85_RS17540 (3606850) 3606850..3611430 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3604623..3639383 34760


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T210507 WP_001094400.1 NZ_CP079992:3606157-3606486 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T210507 NZ_CP104533:2539271-2539549 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT210507 WP_000072690.1 NZ_CP079992:3605819-3606136 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT210507 NZ_CP104533:2539549-2539833 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References