Detailed information of TA system TA210491

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 1154565..1155244
• Replicon: chromosome
• Accession: NZ_CP079992
• Organism: Escherichia coli strain KMS2001

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: P77692
• Locus tag: KYK85_RS05540
• Protein ID: WP_000854672.1
• Coordinates: 1154565..1154906 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: Q47684
• Locus tag: KYK85_RS05545
• Protein ID: WP_000070395.1
• Coordinates: 1154927..1155244 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYK85_RS05515 (1149842)	1149842..1150243	+	402	WP_000174689.1	sigma factor-binding protein Crl	-
KYK85_RS05520 (1150282)	1150282..1151337	-	1056	WP_000749863.1	phosphoporin PhoE	-
KYK85_RS05525 (1151625)	1151625..1152728	+	1104	WP_001285288.1	glutamate 5-kinase	-
KYK85_RS05530 (1152740)	1152740..1153993	+	1254	WP_000893278.1	glutamate-5-semialdehyde dehydrogenase	-
KYK85_RS05540 (1154565)	1154565..1154906	-	342	WP_000854672.1	type IV toxin-antitoxin system toxin YkfI	Toxin
KYK85_RS05545 (1154927)	1154927..1155244	-	318	WP_000070395.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
KYK85_RS05550 (1155263)	1155263..1155484	-	222	WP_000691994.1	DUF987 domain-containing protein	-
KYK85_RS05555 (1155493)	1155493..1155969	-	477	WP_000811693.1	RadC family protein	-
KYK85_RS05560 (1155985)	1155985..1156443	-	459	WP_000211838.1	antirestriction protein	-
KYK85_RS05565 (1156541)	1156541..1156780	-	240	WP_000194654.1	DUF905 family protein	-
KYK85_RS05570 (1156857)	1156857..1157324	-	468	WP_001547765.1	protein YkfB	-
KYK85_RS05575 (1157347)	1157347..1157790	-	444	WP_000824223.1	lipoprotein YafY	-
KYK85_RS05580 (1157790)	1157790..1158017	-	228	WP_001548158.1	protein YpjK	-
KYK85_RS05585 (1158013)	1158013..1158204	-	192	Protein_1097	DeoR family transcriptional regulator	-
KYK85_RS05590 (1158421)	1158421..1159242	-	822	WP_000197389.1	DUF932 domain-containing protein	-
KYK85_RS05595 (1159334)	1159334..1160197	-	864	WP_001065553.1	GTPase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12905.01 Da
• Isoelectric Point: 9.6543

>T210491 WP_000854672.1 NZ_CP079992:c1154906-1154565 [Escherichia coli]
MKTLPAITQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T210491 NZ_CP104526:c4114413-4114126 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11939.59 Da
• Isoelectric Point: 6.7391

>AT210491 WP_000070395.1 NZ_CP079992:c1155244-1154927 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFILQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT210491 NZ_CP104526:c4114139-4113837 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTTATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: P77692

Antitoxin

• AlphaFold DB: Q47684