Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3895011..3895665 Replicon chromosome
Accession NZ_CP079991
Organism Escherichia coli strain KMS2005

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag KYK86_RS18845 Protein ID WP_000244781.1
Coordinates 3895011..3895418 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KYK86_RS18850 Protein ID WP_000354046.1
Coordinates 3895399..3895665 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK86_RS18825 (3890968) 3890968..3892701 - 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -
KYK86_RS18830 (3892707) 3892707..3893417 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KYK86_RS18835 (3893442) 3893442..3894338 - 897 WP_000806987.1 site-specific tyrosine recombinase XerD -
KYK86_RS18840 (3894450) 3894450..3894971 + 522 WP_001055874.1 flavodoxin FldB -
KYK86_RS18845 (3895011) 3895011..3895418 - 408 WP_000244781.1 protein YgfX Toxin
KYK86_RS18850 (3895399) 3895399..3895665 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KYK86_RS18855 (3895908) 3895908..3896888 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
KYK86_RS18860 (3896965) 3896965..3897624 - 660 WP_000250274.1 hemolysin III family protein -
KYK86_RS18865 (3897788) 3897788..3898099 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
KYK86_RS18870 (3898144) 3898144..3899577 + 1434 WP_001350137.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T210477 WP_000244781.1 NZ_CP079991:c3895418-3895011 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T210477 NZ_CP104526:192951-193058 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTTGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT210477 WP_000354046.1 NZ_CP079991:c3895665-3895399 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT210477 NZ_CP104526:c192903-192837 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References