Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3741140..3741723 Replicon chromosome
Accession NZ_CP079991
Organism Escherichia coli strain KMS2005

Toxin (Protein)


Gene name mazF Uniprot ID V0SV58
Locus tag KYK86_RS18215 Protein ID WP_000254750.1
Coordinates 3741140..3741475 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag KYK86_RS18220 Protein ID WP_000581937.1
Coordinates 3741475..3741723 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK86_RS18200 (3737026) 3737026..3738324 - 1299 WP_000036723.1 phosphopyruvate hydratase -
KYK86_RS18205 (3738412) 3738412..3740049 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
KYK86_RS18210 (3740277) 3740277..3741068 - 792 WP_001071651.1 nucleoside triphosphate pyrophosphohydrolase -
KYK86_RS18215 (3741140) 3741140..3741475 - 336 WP_000254750.1 endoribonuclease MazF Toxin
KYK86_RS18220 (3741475) 3741475..3741723 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
KYK86_RS18225 (3741801) 3741801..3744035 - 2235 WP_000226815.1 GTP pyrophosphokinase -
KYK86_RS18230 (3744083) 3744083..3745384 - 1302 WP_000046816.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12067.95 Da        Isoelectric Point: 8.2618

>T210476 WP_000254750.1 NZ_CP079991:c3741475-3741140 [Escherichia coli]
MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T210476 NZ_CP104525:63880-64032 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT210476 WP_000581937.1 NZ_CP079991:c3741723-3741475 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT210476 NZ_CP104525:c63825-63768 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SV58

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References