Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 3605818..3606485 Replicon chromosome
Accession NZ_CP079991
Organism Escherichia coli strain KMS2005

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag KYK86_RS17555 Protein ID WP_001094400.1
Coordinates 3606156..3606485 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag KYK86_RS17550 Protein ID WP_000072690.1
Coordinates 3605818..3606135 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK86_RS17520 (3600870) 3600870..3601879 - 1010 Protein_3441 arsenic transporter -
KYK86_RS17525 (3602021) 3602021..3603724 + 1704 WP_000896263.1 protein YfjW -
KYK86_RS17530 (3604335) 3604335..3604519 + 185 Protein_3443 DUF905 family protein -
KYK86_RS17535 (3604622) 3604622..3605080 + 459 WP_000211841.1 antirestriction protein -
KYK86_RS17540 (3605089) 3605089..3605571 + 483 WP_240275544.1 RadC family protein -
KYK86_RS17545 (3605580) 3605580..3605780 + 201 WP_001395452.1 DUF987 domain-containing protein -
KYK86_RS17550 (3605818) 3605818..3606135 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KYK86_RS17555 (3606156) 3606156..3606485 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
KYK86_RS17560 (3606849) 3606849..3611429 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T210475 WP_001094400.1 NZ_CP079991:3606156-3606485 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T210475 NZ_CP104525:2255-2533 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT210475 WP_000072690.1 NZ_CP079991:3605818-3606135 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT210475 NZ_CP104525:1986-2255 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References