Detailed information of TA system TA210474

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 3594621..3596058
• Replicon: chromosome
• Accession: NZ_CP079991
• Organism: Escherichia coli strain KMS2005

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: P52129
• Locus tag: KYK86_RS17475
• Protein ID: WP_000155570.1
• Coordinates: 3594621..3595694 (+)
• Length: 358 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: P52130
• Locus tag: KYK86_RS17480
• Protein ID: WP_000461704.1
• Coordinates: 3595687..3596058 (+)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYK86_RS17460 (3590054)	3590054..3592243	-	2190	WP_000136402.1	DEAD/DEAH box helicase	-
KYK86_RS17465 (3592240)	3592240..3593856	-	1617	WP_000445380.1	anti-bacteriophage protein AbpA	-
KYK86_RS17470 (3594216)	3594216..3594479	-	264	WP_000502050.1	DUF1524 domain-containing protein	-
KYK86_RS17475 (3594621)	3594621..3595694	+	1074	WP_000155570.1	type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA	Toxin
KYK86_RS17480 (3595687)	3595687..3596058	+	372	WP_000461704.1	type II toxin-antitoxin system antitoxin RnlB	Antitoxin
KYK86_RS17485 (3596413)	3596413..3597276	+	864	WP_000787597.1	GTPase family protein	-
KYK86_RS17490 (3597368)	3597368..3598189	+	822	WP_000197391.1	DUF932 domain-containing protein	-
KYK86_RS17495 (3598406)	3598406..3599107	+	702	WP_000189409.1	WYL domain-containing protein	-
KYK86_RS17500 (3599148)	3599148..3599384	+	237	WP_001144029.1	protein YpjK	-
KYK86_RS17505 (3599384)	3599384..3599827	+	444	WP_000824222.1	lipoprotein YfjS	-
KYK86_RS17510 (3599851)	3599851..3600318	+	468	WP_000702495.1	protein YfjT	-
KYK86_RS17515 (3600705)	3600705..3600857	-	153	Protein_3440	arsenate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (14-111)

Sequences

Toxin

Protein

• Length: 358 a.a.
• Molecular weight: 40061.13 Da
• Isoelectric Point: 6.8293

>T210474 WP_000155570.1 NZ_CP079991:3594621-3595694 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Nucleotide

• Length: 1074 bp

>T210474 NZ_CP104524:c53701-53423 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13664.69 Da
• Isoelectric Point: 4.2391

>AT210474 WP_000461704.1 NZ_CP079991:3595687-3596058 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Nucleotide

• Length: 372 bp

>AT210474 NZ_CP104524:c53970-53701 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• PDB: 6Y2R
• PDB: 7AEX
• PDB: 6Y2Q
• PDB: 4I8O
• PDB: 6Y2P
• PDB: 6Y2S

Antitoxin

• PDB: 6Y2P
• AlphaFold DB: A0A373F4G4