Detailed information of TA system TA210449

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 42759..43363
• Replicon: plasmid p19Msa0383-1
• Accession: NZ_CP079985
• Organism: Macrococcus bohemicus strain 19Msa0383

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: KYI13_RS12555
• Protein ID: WP_219499250.1
• Coordinates: 43016..43363 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: KYI13_RS12550
• Protein ID: WP_219499246.1
• Coordinates: 42759..43022 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYI13_RS12535 (KYI13_12540)	38562..41732	+	3171	WP_219499239.1	hypothetical protein	-
KYI13_RS12540 (KYI13_12545)	41775..42095	+	321	WP_219499241.1	DUF5592 family protein	-
KYI13_RS12545 (KYI13_12550)	42100..42687	+	588	WP_219499243.1	hypothetical protein	-
KYI13_RS12550 (KYI13_12555)	42759..43022	+	264	WP_219499246.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KYI13_RS12555 (KYI13_12560)	43016..43363	+	348	WP_219499250.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KYI13_RS12560 (KYI13_12565)	43547..45511	+	1965	WP_219499253.1	hypothetical protein	-
KYI13_RS12565 (KYI13_12570)	45529..45996	+	468	WP_219499256.1	hypothetical protein	-
KYI13_RS12570 (KYI13_12575)	46052..47335	+	1284	WP_244999807.1	glucosaminidase domain-containing protein	-
KYI13_RS12575 (KYI13_12580)	47355..47966	+	612	WP_219499259.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..102209	102209

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-90)

Antitoxin

• SpoVT-AbrB domain (11-54)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13167.23 Da
• Isoelectric Point: 7.4005

>T210449 WP_219499250.1 NZ_CP079985:43016-43363 [Macrococcus bohemicus]
MVEEVSQGDIFYVNFNPRKGHEQKDKRPALAISHDMVRDTSNLTIVVPISNTKRSFPMYHTLKTTKKIEGKVLLDQSIAL
DLKARGISKDNVVEIIDKEELSEIIHLYKLLFTVD

Nucleotide

• Length: 348 bp

>T210449 NZ_CP104523:c292796-292419 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCAACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACAATGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10111.47 Da
• Isoelectric Point: 4.8839

>AT210449 WP_219499246.1 NZ_CP079985:42759-43022 [Macrococcus bohemicus]
MSTLETTTTLKKWGNSKGLRLPQKVITHLNLHEDQKILIVLEEDKLILKPQTEKPTNIHELFSGWKDDGIRSEEMDWGQA
EGEELNW

Nucleotide

• Length: 264 bp

>AT210449 NZ_CP104523:c293220-292843 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCATCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGATACCCTTGGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAATCACCGTATAA