Detailed information of TA system TA210426

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 4478331..4478947
• Replicon: chromosome
• Accession: NZ_CP079939
• Organism: Enterobacter hormaechei strain G17

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KY275_RS23290
• Protein ID: WP_080336832.1
• Coordinates: 4478331..4478702 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: KY275_RS23295
• Protein ID: WP_015569912.1
• Coordinates: 4478705..4478947 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KY275_RS23275 (KY275_23280)	4475831..4476733	+	903	WP_014072386.1	formate dehydrogenase subunit beta	-
KY275_RS23280 (KY275_23285)	4476730..4477365	+	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
KY275_RS23285 (KY275_23290)	4477362..4478291	+	930	WP_022652077.1	formate dehydrogenase accessory protein FdhE	-
KY275_RS23290 (KY275_23295)	4478331..4478702	-	372	WP_080336832.1	type II toxin-antitoxin system VapC family toxin	Toxin
KY275_RS23295 (KY275_23300)	4478705..4478947	-	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
KY275_RS23300 (KY275_23305)	4479146..4480066	+	921	WP_213789396.1	alpha/beta hydrolase	-
KY275_RS23305 (KY275_23310)	4480075..4481016	-	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
KY275_RS23310 (KY275_23315)	4481061..4481498	-	438	WP_213789397.1	D-aminoacyl-tRNA deacylase	-
KY275_RS23315 (KY275_23320)	4481495..4482376	-	882	WP_003861949.1	virulence factor BrkB family protein	-
KY275_RS23320 (KY275_23325)	4482370..4482969	-	600	WP_032649898.1	glucose-1-phosphatase	-
KY275_RS23325 (KY275_23330)	4483088..4483888	-	801	WP_003861944.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13737.88 Da
• Isoelectric Point: 6.4882

>T210426 WP_080336832.1 NZ_CP079939:c4478702-4478331 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQL

Nucleotide

• Length: 372 bp

>T210426 NZ_CP104521:c824424-824047 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT210426 WP_015569912.1 NZ_CP079939:c4478947-4478705 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT210426 NZ_CP104521:c824845-824471 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3