Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 550132..550703 Replicon chromosome
Accession NZ_CP079927
Organism Enterococcus faecium strain 7150

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag KYK17_RS02635 Protein ID WP_002286801.1
Coordinates 550362..550703 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag KYK17_RS02630 Protein ID WP_002323011.1
Coordinates 550132..550362 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYK17_RS02600 (KYK17_02600) 545265..546596 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
KYK17_RS02605 (KYK17_02605) 546618..547244 + 627 WP_002286816.1 cysteine hydrolase -
KYK17_RS02610 (KYK17_02610) 547427..548008 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
KYK17_RS13440 548740..549302 + 563 Protein_519 SOS response-associated peptidase -
KYK17_RS02625 (KYK17_02625) 549507..549845 - 339 WP_002286804.1 hypothetical protein -
KYK17_RS02630 (KYK17_02630) 550132..550362 + 231 WP_002323011.1 hypothetical protein Antitoxin
KYK17_RS02635 (KYK17_02635) 550362..550703 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KYK17_RS02640 (KYK17_02640) 551553..551738 + 186 WP_002304207.1 hypothetical protein -
KYK17_RS02645 (KYK17_02645) 552243..552437 + 195 WP_002297028.1 hypothetical protein -
KYK17_RS02650 (KYK17_02650) 552628..552877 + 250 Protein_525 transposase -
KYK17_RS02655 (KYK17_02655) 553121..553951 - 831 WP_002286789.1 manganese catalase family protein -
KYK17_RS02660 (KYK17_02660) 554159..555493 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T210406 WP_002286801.1 NZ_CP079927:550362-550703 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T210406 NZ_CP104516:2063973-2064251 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAA
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTATTTGGGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT210406 WP_002323011.1 NZ_CP079927:550132-550362 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT210406 NZ_CP104516:2063710-2063970 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAAGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATTAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References