Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3394977..3395595 Replicon chromosome
Accession NZ_CP079924
Organism Escherichia sp. TC-EC600-tetX4

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KXD89_RS16500 Protein ID WP_001291435.1
Coordinates 3395377..3395595 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KXD89_RS16495 Protein ID WP_000344800.1
Coordinates 3394977..3395351 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXD89_RS16485 (3390066) 3390066..3391259 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KXD89_RS16490 (3391282) 3391282..3394431 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KXD89_RS16495 (3394977) 3394977..3395351 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KXD89_RS16500 (3395377) 3395377..3395595 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KXD89_RS16505 (3395767) 3395767..3396318 + 552 WP_000102564.1 maltose O-acetyltransferase -
KXD89_RS16510 (3396434) 3396434..3396904 + 471 WP_000136192.1 YlaC family protein -
KXD89_RS16515 (3397068) 3397068..3398618 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KXD89_RS16520 (3398660) 3398660..3399013 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KXD89_RS16530 (3399392) 3399392..3399703 + 312 WP_000409911.1 MGMT family protein -
KXD89_RS16535 (3399734) 3399734..3400306 - 573 WP_000779842.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T210393 WP_001291435.1 NZ_CP079924:3395377-3395595 [Escherichia sp. TC-EC600-tetX4]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T210393 NZ_CP104511:28161-28439 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAACGGTCGTG
TGGAAGGAACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
GTCCTGCGCGTGTTGCATACCGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT210393 WP_000344800.1 NZ_CP079924:3394977-3395351 [Escherichia sp. TC-EC600-tetX4]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT210393 NZ_CP104511:27892-28161 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCCCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCACGGCGATGTGCTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References