Detailed information of TA system TA210392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2369219..2369857
• Replicon: chromosome
• Accession: NZ_CP079924
• Organism: Escherichia sp. TC-EC600-tetX4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KXD89_RS11460
• Protein ID: WP_000813794.1
• Coordinates: 2369681..2369857 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KXD89_RS11455
• Protein ID: WP_001270286.1
• Coordinates: 2369219..2369635 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXD89_RS11435 (2364371)	2364371..2365312	-	942	WP_001251304.1	ABC transporter permease	-
KXD89_RS11440 (2365313)	2365313..2366326	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
KXD89_RS11445 (2366344)	2366344..2367489	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KXD89_RS11450 (2367734)	2367734..2369140	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KXD89_RS11455 (2369219)	2369219..2369635	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KXD89_RS11460 (2369681)	2369681..2369857	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KXD89_RS11465 (2370079)	2370079..2370309	+	231	WP_000494244.1	YncJ family protein	-
KXD89_RS11470 (2370401)	2370401..2372362	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KXD89_RS11475 (2372435)	2372435..2372971	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KXD89_RS11480 (2373063)	2373063..2374238	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T210392 WP_000813794.1 NZ_CP079924:c2369857-2369681 [Escherichia sp. TC-EC600-tetX4]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T210392 NZ_CP104511:2060-2347 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT210392 WP_001270286.1 NZ_CP079924:c2369635-2369219 [Escherichia sp. TC-EC600-tetX4]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT210392 NZ_CP104511:1812-2063 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCCTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Structures

Toxin

• PDB: 6HPB