Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2233271..2233797 Replicon chromosome
Accession NZ_CP079924
Organism Escherichia sp. TC-EC600-tetX4

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag KXD89_RS10780 Protein ID WP_000323025.1
Coordinates 2233510..2233797 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag KXD89_RS10775 Protein ID WP_000534858.1
Coordinates 2233271..2233510 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXD89_RS10720 (2228298) 2228298..2229065 - 768 Protein_2095 exonuclease -
KXD89_RS10725 (2229158) 2229158..2229349 - 192 WP_001083297.1 lysis protein YdfD -
KXD89_RS10730 (2229346) 2229346..2229534 - 189 WP_000854559.1 cell division inhibition protein DicB -
KXD89_RS10735 (2230102) 2230102..2230320 - 219 WP_001171942.1 protein YdfC -
KXD89_RS22305 (2230350) 2230350..2230478 - 129 WP_000344964.1 protein YdfB -
KXD89_RS10740 (2230480) 2230480..2230635 - 156 WP_000379575.1 DUF1391 family protein -
KXD89_RS10745 (2230802) 2230802..2231209 - 408 WP_000448564.1 DNA-binding transcriptional dual regulator DicA -
KXD89_RS10750 (2231293) 2231293..2231523 + 231 WP_000920568.1 dicB transcriptional regulator DicC -
KXD89_RS10755 (2231507) 2231507..2231785 + 279 Protein_2103 hypothetical protein -
KXD89_RS10760 (2231820) 2231820..2231969 + 150 WP_011443592.1 protein YdfW -
KXD89_RS10765 (2232406) 2232406..2232738 - 333 WP_001301033.1 FlxA-like family protein -
KXD89_RS10770 (2232941) 2232941..2233246 - 306 WP_001326990.1 protein YdfV -
KXD89_RS10775 (2233271) 2233271..2233510 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
KXD89_RS10780 (2233510) 2233510..2233797 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
KXD89_RS10785 (2233869) 2233869..2234024 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
KXD89_RS10790 (2234241) 2234241..2234492 + 252 WP_000980994.1 protein Rem -
KXD89_RS10795 (2234559) 2234559..2234837 + 279 WP_012304870.1 hypothetical protein -
KXD89_RS10800 (2234839) 2234839..2235888 + 1050 WP_001265198.1 DUF968 domain-containing protein -
KXD89_RS10805 (2235902) 2235902..2236654 + 753 WP_001047135.1 antitermination protein -
KXD89_RS10810 (2236932) 2236932..2237021 - 90 WP_120795389.1 hypothetical protein -
KXD89_RS10815 (2237076) 2237076..2237288 - 213 WP_000087756.1 cold shock-like protein CspF -
KXD89_RS10820 (2237589) 2237589..2237804 + 216 WP_000066484.1 cold shock-like protein CspB -
KXD89_RS10825 (2238168) 2238168..2238338 - 171 WP_211180497.1 putative zinc-binding protein YnfU -
KXD89_RS10830 (2238558) 2238558..2238773 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2226435..2250791 24356


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T210390 WP_000323025.1 NZ_CP079924:2233510-2233797 [Escherichia sp. TC-EC600-tetX4]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T210390 NZ_CP104509:c4727909-4727802 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT210390 WP_000534858.1 NZ_CP079924:2233271-2233510 [Escherichia sp. TC-EC600-tetX4]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT210390 NZ_CP104509:4727957-4728023 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References