Detailed information of TA system TA210381

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1801976..1802807
• Replicon: chromosome
• Accession: NZ_CP079924
• Organism: Escherichia sp. TC-EC600-tetX4

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: KXD89_RS08565
• Protein ID: WP_000854814.1
• Coordinates: 1801976..1802350 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: P76364
• Locus tag: KXD89_RS08570
• Protein ID: WP_001285584.1
• Coordinates: 1802439..1802807 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXD89_RS08525 (1797372)	1797372..1798538	+	1167	WP_000830156.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-
KXD89_RS08530 (1798657)	1798657..1799130	+	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-
KXD89_RS08535 (1799328)	1799328..1800386	+	1059	WP_001200891.1	FUSC family protein	-
KXD89_RS08540 (1800558)	1800558..1800887	+	330	WP_000450409.1	DUF496 family protein	-
KXD89_RS08545 (1800988)	1800988..1801122	-	135	WP_001297944.1	EutP/PduV family microcompartment system protein	-
KXD89_RS08550 (1801242)	1801242..1801370	+	129	Protein_1672	transposase domain-containing protein	-
KXD89_RS08555 (1801659)	1801659..1801739	-	81	Protein_1673	hypothetical protein	-
KXD89_RS08560 (1801785)	1801785..1801979	-	195	WP_000988600.1	DUF5983 family protein	-
KXD89_RS08565 (1801976)	1801976..1802350	-	375	WP_000854814.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KXD89_RS08570 (1802439)	1802439..1802807	-	369	WP_001285584.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
KXD89_RS08575 (1802881)	1802881..1803102	-	222	WP_000692323.1	DUF987 domain-containing protein	-
KXD89_RS08580 (1803165)	1803165..1803611	-	447	WP_000187523.1	RadC family protein	-
KXD89_RS08585 (1803608)	1803608..1805140	-	1533	WP_001350525.1	protein YeeR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T210381 WP_000854814.1 NZ_CP079924:c1802350-1801976 [Escherichia sp. TC-EC600-tetX4]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T210381 NZ_CP104509:c2942039-2941785 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT210381 WP_001285584.1 NZ_CP079924:c1802807-1802439 [Escherichia sp. TC-EC600-tetX4]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT210381 NZ_CP104509:c2942287-2942036 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• PDB: 2H28
• AlphaFold DB: A0A1M2E8G6