Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 1107986..1108653 Replicon chromosome
Accession NZ_CP079924
Organism Escherichia sp. TC-EC600-tetX4

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag KXD89_RS05315 Protein ID WP_001094400.1
Coordinates 1107986..1108315 (-) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag KXD89_RS05320 Protein ID WP_000072690.1
Coordinates 1108336..1108653 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KXD89_RS05310 (1103042) 1103042..1107622 + 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -
KXD89_RS05315 (1107986) 1107986..1108315 - 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
KXD89_RS05320 (1108336) 1108336..1108653 - 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
KXD89_RS05325 (1108691) 1108691..1108891 - 201 WP_001395452.1 DUF987 domain-containing protein -
KXD89_RS05330 (1108900) 1108900..1109382 - 483 WP_001407480.1 RadC family protein -
KXD89_RS05335 (1109391) 1109391..1109849 - 459 WP_000211841.1 antirestriction protein -
KXD89_RS05340 (1109952) 1109952..1110136 - 185 Protein_1042 DUF905 domain-containing protein -
KXD89_RS05345 (1110747) 1110747..1112450 - 1704 WP_000896263.1 protein YfjW -
KXD89_RS05350 (1112592) 1112592..1113601 + 1010 Protein_1044 arsenic transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T210378 WP_001094400.1 NZ_CP079924:c1108315-1107986 [Escherichia sp. TC-EC600-tetX4]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T210378 NZ_CP104509:2321973-2322149 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT210378 WP_000072690.1 NZ_CP079924:c1108653-1108336 [Escherichia sp. TC-EC600-tetX4]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT210378 NZ_CP104509:2322195-2322611 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References