Detailed information of TA system TA210363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1780404..1781032
• Replicon: chromosome
• Accession: NZ_CP079921
• Organism: Actinobacillus pleuropneumoniae strain P1875

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KZH40_RS08345
• Protein ID: WP_005602539.1
• Coordinates: 1780404..1780802 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3S5F5Z7
• Locus tag: KZH40_RS08350
• Protein ID: WP_005602537.1
• Coordinates: 1780802..1781032 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZH40_RS08320 (KZH40_08370)	1775935..1776456	+	522	WP_005613306.1	ATP-dependent protease subunit HslV	-
KZH40_RS08325 (KZH40_08375)	1776602..1777924	+	1323	WP_005620148.1	HslU--HslV peptidase ATPase subunit	-
KZH40_RS08330 (KZH40_08380)	1777997..1778776	+	780	WP_005602546.1	hypothetical protein	-
KZH40_RS08335 (KZH40_08385)	1778956..1779606	-	651	WP_005602543.1	DUF2202 domain-containing protein	-
KZH40_RS08340 (KZH40_08390)	1779852..1780268	+	417	WP_005602541.1	DUF417 family protein	-
KZH40_RS08345 (KZH40_08395)	1780404..1780802	-	399	WP_005602539.1	tRNA(fMet)-specific endonuclease VapC	Toxin
KZH40_RS08350 (KZH40_08400)	1780802..1781032	-	231	WP_005602537.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KZH40_RS08355 (KZH40_08405)	1781140..1781280	+	141	WP_005602535.1	hypothetical protein	-
KZH40_RS08360 (KZH40_08410)	1781396..1782178	-	783	WP_005602534.1	HincII family type II restriction endonuclease	-
KZH40_RS08365 (KZH40_08415)	1782175..1783725	-	1551	WP_005602533.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1756093..1796335	40242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (6-53)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15003.30 Da
• Isoelectric Point: 8.0810

>T210363 WP_005602539.1 NZ_CP079921:c1780802-1780404 [Actinobacillus pleuropneumoniae]
MLAYMLDTNIAIYVIKRRPIEVLDKFNLNSTRLCVSSITAAELYYGAEKSQFPERNMAVIEDFLSRLTILDYTHKAATHF
GNIKAHLSKQGKIIGENDIHIAAHARSEGLVLVTNNLRAFERVEGLRLDNWV

Nucleotide

• Length: 399 bp

>T210363 NZ_CP104503:c4479286-4479068 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8591.74 Da
• Isoelectric Point: 4.7063

>AT210363 WP_005602537.1 NZ_CP079921:c1781032-1780802 [Actinobacillus pleuropneumoniae]
MEASVFMTNRSQAVRLPAEVRFASDVKKLSVRVVGNERILAPINQSWDSFFLSSATVSDDFMTEREISIQPEREGL

Nucleotide

• Length: 231 bp

>AT210363 NZ_CP104503:c4479686-4479312 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A3S5F5Z7