Detailed information of TA system TA210362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-YefM
• Location: 1683196..1683911
• Replicon: chromosome
• Accession: NZ_CP079921
• Organism: Actinobacillus pleuropneumoniae strain P1875

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KZH40_RS07770
• Protein ID: WP_005599445.1
• Coordinates: 1683546..1683911 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B0BT01
• Locus tag: KZH40_RS07765
• Protein ID: WP_005599447.1
• Coordinates: 1683196..1683483 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KZH40_RS07740 (KZH40_07795)	1678799..1679392	-	594	WP_005599453.1	protein-methionine-sulfoxide reductase heme-binding subunit MsrQ	-
KZH40_RS07745 (KZH40_07800)	1679392..1680348	-	957	WP_005619040.1	protein-methionine-sulfoxide reductase catalytic subunit MsrP	-
KZH40_RS07750 (KZH40_07805)	1680437..1681018	+	582	WP_005599450.1	sigma-70 family RNA polymerase sigma factor	-
KZH40_RS07755 (KZH40_07810)	1681021..1681209	+	189	WP_005602684.1	hypothetical protein	-
KZH40_RS07760 (KZH40_07815)	1681275..1682990	-	1716	WP_005602682.1	proline--tRNA ligase	-
KZH40_RS07765 (KZH40_07820)	1683196..1683483	+	288	WP_005599447.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KZH40_RS07770 (KZH40_07825)	1683546..1683911	+	366	WP_005599445.1	type II toxin-antitoxin system VapC family toxin	Toxin
KZH40_RS07775 (KZH40_07830)	1684035..1686116	+	2082	WP_005602680.1	ATP-dependent DNA helicase RecG	-
KZH40_RS07780 (KZH40_07835)	1686243..1686524	+	282	WP_005599441.1	DNA-directed RNA polymerase subunit omega	-
KZH40_RS07785 (KZH40_07840)	1686620..1688326	+	1707	WP_005602678.1	bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13974.06 Da
• Isoelectric Point: 9.0549

>T210362 WP_005599445.1 NZ_CP079921:1683546-1683911 [Actinobacillus pleuropneumoniae]
MDTNVRQWLATIKPSQTNISCITLSEIKTGILLKARKDPIQAERLNHWFTHNVLSVYQAKSFSINNEIALLASEYHIPNK
MDLNDAYIAATAKYHNLVLVTRNLKDFNRCDIRLFNPFEPN

Nucleotide

• Length: 366 bp

>T210362 NZ_CP104503:4243430-4243828 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGAAGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11040.29 Da
• Isoelectric Point: 4.5578

>AT210362 WP_005599447.1 NZ_CP079921:1683196-1683483 [Actinobacillus pleuropneumoniae]
MPITITSREFNQHASKVLKMAEKEPVFITKWGKIVSVVSNYVDYKQHTTVEPSFEELFASSTDESVSDEFWQDFEQTLTK
IRKSNKSEQIDFGDE

Nucleotide

• Length: 288 bp

>AT210362 NZ_CP104503:4243134-4243427 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTTGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAACTGGTTAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A3N3C5