Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-DnaT |
| Location | 13898..14420 | Replicon | plasmid p35ZF15-2-1 |
| Accession | NZ_CP079894 | ||
| Organism | Escherichia fergusonii strain 5zf15-2-1 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | G3CAN5 |
| Locus tag | KYD87_RS25370 | Protein ID | WP_000220560.1 |
| Coordinates | 14139..14420 (+) | Length | 94 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | G3CAG1 |
| Locus tag | KYD87_RS25365 | Protein ID | WP_000121743.1 |
| Coordinates | 13898..14149 (+) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KYD87_RS25335 (KYD87_25195) | 9261..9491 | + | 231 | WP_000051064.1 | plasmid partition protein ParG | - |
| KYD87_RS25340 (KYD87_25200) | 9507..9800 | + | 294 | WP_015060510.1 | hypothetical protein | - |
| KYD87_RS25345 (KYD87_25205) | 9802..10770 | + | 969 | WP_166501310.1 | IS5-like element IS903B family transposase | - |
| KYD87_RS25350 (KYD87_25210) | 10830..11183 | - | 354 | WP_000876434.1 | DNA distortion polypeptide 3 | - |
| KYD87_RS25355 (KYD87_25215) | 11320..11766 | - | 447 | WP_001074386.1 | hypothetical protein | - |
| KYD87_RS25360 (KYD87_25220) | 11806..12642 | - | 837 | WP_001050931.1 | RepB family plasmid replication initiator protein | - |
| KYD87_RS25365 (KYD87_25225) | 13898..14149 | + | 252 | WP_000121743.1 | plasmid stabilization protein | Antitoxin |
| KYD87_RS25370 (KYD87_25230) | 14139..14420 | + | 282 | WP_000220560.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| KYD87_RS25375 (KYD87_25235) | 14566..14889 | + | 324 | WP_001181903.1 | hypothetical protein | - |
| KYD87_RS25380 (KYD87_25240) | 14934..15179 | + | 246 | WP_000356546.1 | hypothetical protein | - |
| KYD87_RS25385 (KYD87_25245) | 15169..15384 | + | 216 | WP_001180116.1 | hypothetical protein | - |
| KYD87_RS25390 (KYD87_25250) | 15477..15806 | + | 330 | WP_000866648.1 | hypothetical protein | - |
| KYD87_RS25395 (KYD87_25255) | 16093..16242 | + | 150 | WP_000003880.1 | hypothetical protein | - |
| KYD87_RS25400 (KYD87_25260) | 16255..16527 | + | 273 | WP_000160399.1 | hypothetical protein | - |
| KYD87_RS25405 (KYD87_25265) | 16854..17399 | + | 546 | WP_038976855.1 | DNA distortion polypeptide 1 | - |
| KYD87_RS25410 (KYD87_25270) | 17402..18559 | + | 1158 | WP_000538023.1 | DNA distortion polypeptide 2 | - |
| KYD87_RS25415 (KYD87_25275) | 18920..19411 | + | 492 | WP_000872475.1 | transcription termination/antitermination NusG family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | - | 1..30790 | 30790 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 11005.83 Da Isoelectric Point: 10.5938
>T210356 WP_000220560.1 NZ_CP079894:14139-14420 [Escherichia fergusonii]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
Download Length: 282 bp
>T210356 NZ_CP104503:3298264-3298575 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9194.43 Da Isoelectric Point: 4.1683
>AT210356 WP_000121743.1 NZ_CP079894:13898-14149 [Escherichia fergusonii]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
Download Length: 252 bp
>AT210356 NZ_CP104503:3298576-3298866 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | G3CAN5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3S5I301 |