Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 4531345..4531970 Replicon chromosome
Accession NZ_CP079891
Organism Escherichia fergusonii strain 5zf15-2-1

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag KYD87_RS22215 Protein ID WP_000911329.1
Coordinates 4531572..4531970 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag KYD87_RS22210 Protein ID WP_000450524.1
Coordinates 4531345..4531572 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYD87_RS22185 (4527146) 4527146..4527616 - 471 WP_001068688.1 thioredoxin-dependent thiol peroxidase -
KYD87_RS22190 (4527616) 4527616..4528188 - 573 WP_182211927.1 glycine cleavage system transcriptional repressor -
KYD87_RS22195 (4528335) 4528335..4529213 + 879 WP_000494011.1 4-hydroxy-tetrahydrodipicolinate synthase -
KYD87_RS22200 (4529230) 4529230..4530264 + 1035 WP_002431657.1 outer membrane protein assembly factor BamC -
KYD87_RS22205 (4530477) 4530477..4531190 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
KYD87_RS22210 (4531345) 4531345..4531572 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
KYD87_RS22215 (4531572) 4531572..4531970 + 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
KYD87_RS22220 (4532117) 4532117..4532980 + 864 WP_001267498.1 neutral zinc metallopeptidase -
KYD87_RS22225 (4532995) 4532995..4535010 + 2016 WP_182211926.1 tRNA cytosine(34) acetyltransferase TmcA -
KYD87_RS22230 (4535084) 4535084..4535782 + 699 WP_000679812.1 esterase -
KYD87_RS22235 (4535875) 4535875..4536075 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T210352 WP_000911329.1 NZ_CP079891:4531572-4531970 [Escherichia fergusonii]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T210352 NZ_CP104503:c2086515-2086108 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTATTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT210352 WP_000450524.1 NZ_CP079891:4531345-4531572 [Escherichia fergusonii]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT210352 NZ_CP104503:c2086762-2086496 [Escherichia coli]
ATGGACATTAATAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References