Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3906351..3906901 Replicon chromosome
Accession NZ_CP079891
Organism Escherichia fergusonii strain 5zf15-2-1

Toxin (Protein)


Gene name ccdB Uniprot ID F0JPU1
Locus tag KYD87_RS19370 Protein ID WP_001160963.1
Coordinates 3906587..3906901 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag KYD87_RS19365 Protein ID WP_000125566.1
Coordinates 3906351..3906584 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYD87_RS19340 (3901423) 3901423..3901710 + 288 WP_001596509.1 ferredoxin-like protein FixX -
KYD87_RS19345 (3901769) 3901769..3903100 + 1332 WP_182210693.1 MFS transporter -
KYD87_RS19350 (3903208) 3903208..3903738 + 531 WP_032183187.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KYD87_RS19355 (3903731) 3903731..3905593 + 1863 WP_104918214.1 glutathione-regulated potassium-efflux system protein KefC -
KYD87_RS19360 (3905786) 3905786..3906265 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
KYD87_RS19365 (3906351) 3906351..3906584 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KYD87_RS19370 (3906587) 3906587..3906901 + 315 WP_001160963.1 CcdB family protein Toxin
KYD87_RS19375 (3906898) 3906898..3907746 - 849 WP_000257179.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KYD87_RS19380 (3907753) 3907753..3908130 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KYD87_RS19385 (3908133) 3908133..3908954 - 822 WP_046081975.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KYD87_RS19390 (3908951) 3908951..3909940 - 990 WP_000241247.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KYD87_RS19395 (3909940) 3909940..3911226 - 1287 WP_000800490.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11722.70 Da        Isoelectric Point: 8.0666

>T210347 WP_001160963.1 NZ_CP079891:3906587-3906901 [Escherichia fergusonii]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T210347 NZ_CP104503:c926420-926318 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT210347 WP_000125566.1 NZ_CP079891:3906351-3906584 [Escherichia fergusonii]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT210347 NZ_CP104503:926280-926548 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB F0JPU1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References