Detailed information of TA system TA210332

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 2381783..2382359
• Replicon: chromosome
• Accession: NZ_CP079891
• Organism: Escherichia fergusonii strain 5zf15-2-1

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A0G9FRD2
• Locus tag: KYD87_RS12035
• Protein ID: WP_001350372.1
• Coordinates: 2382072..2382359 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: KYD87_RS12030
• Protein ID: WP_139470116.1
• Coordinates: 2381783..2382085 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYD87_RS12010 (2377597)	2377597..2378109	+	513	WP_001175455.1	4-hydroxyphenylacetate 3-monooxygenase reductase subunit	-
KYD87_RS12015 (2378379)	2378379..2380529	+	2151	WP_001314407.1	pyruvate/proton symporter BtsT	-
KYD87_RS12020 (2380579)	2380579..2380782	+	204	WP_000467859.1	YbdD/YjiX family protein	-
KYD87_RS12025 (2380793)	2380793..2381749	+	957	WP_046081298.1	GTPase	-
KYD87_RS12030 (2381783)	2381783..2382085	-	303	WP_139470116.1	BrnA antitoxin family protein	Antitoxin
KYD87_RS12035 (2382072)	2382072..2382359	-	288	WP_001350372.1	BrnT family toxin	Toxin
KYD87_RS12040 (2382556)	2382556..2383722	+	1167	WP_000800829.1	restriction endonuclease	-
KYD87_RS12045 (2383786)	2383786..2385405	+	1620	WP_223666417.1	class I SAM-dependent DNA methyltransferase	-
KYD87_RS12050 (2385395)	2385395..2386774	+	1380	WP_046081296.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11209.68 Da
• Isoelectric Point: 7.4020

>T210332 WP_001350372.1 NZ_CP079891:c2382359-2382072 [Escherichia fergusonii]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Nucleotide

• Length: 288 bp

>T210332 NZ_CP104500:3823438-3823980 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11468.94 Da
• Isoelectric Point: 8.0217

>AT210332 WP_139470116.1 NZ_CP079891:c2382085-2381783 [Escherichia fergusonii]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLCDQNKK

Nucleotide

• Length: 303 bp

>AT210332 NZ_CP104500:3823143-3823454 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A0G9FRD2