Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2072054..2072708 Replicon chromosome
Accession NZ_CP079891
Organism Escherichia fergusonii strain 5zf15-2-1

Toxin (Protein)


Gene name cptA Uniprot ID F0JPE9
Locus tag KYD87_RS10540 Protein ID WP_000244774.1
Coordinates 2072054..2072461 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KYD87_RS10545 Protein ID WP_000354046.1
Coordinates 2072442..2072708 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYD87_RS10520 (2068011) 2068011..2069744 - 1734 WP_000813234.1 single-stranded-DNA-specific exonuclease RecJ -
KYD87_RS10525 (2069750) 2069750..2070460 - 711 WP_046076902.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KYD87_RS10530 (2070485) 2070485..2071381 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KYD87_RS10535 (2071493) 2071493..2072014 + 522 WP_001055874.1 flavodoxin FldB -
KYD87_RS10540 (2072054) 2072054..2072461 - 408 WP_000244774.1 protein YgfX Toxin
KYD87_RS10545 (2072442) 2072442..2072708 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KYD87_RS10550 (2072960) 2072960..2073940 + 981 WP_000886103.1 tRNA-modifying protein YgfZ -
KYD87_RS10555 (2074017) 2074017..2074676 - 660 WP_000250283.1 hemolysin III family protein -
KYD87_RS10560 (2074840) 2074840..2075151 - 312 WP_001182962.1 N(4)-acetylcytidine aminohydrolase -
KYD87_RS10565 (2075196) 2075196..2076629 + 1434 WP_024256637.1 6-phospho-beta-glucosidase BglA -
KYD87_RS10570 (2076677) 2076677..2077570 - 894 WP_000819362.1 transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16078.05 Da        Isoelectric Point: 11.2511

>T210330 WP_000244774.1 NZ_CP079891:c2072461-2072054 [Escherichia fergusonii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T210330 NZ_CP104500:c2803388-2803285 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTTTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT210330 WP_000354046.1 NZ_CP079891:c2072708-2072442 [Escherichia fergusonii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT210330 NZ_CP104500:2803284-2803518 [Escherichia coli]
TAAAAAAAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCA
TTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAAT
AAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCGTTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L6ZX35

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References