Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3941884..3942460 Replicon chromosome
Accession NZ_CP079884
Organism Escherichia fergusonii strain 6S41-1

Toxin (Protein)


Gene name shpA Uniprot ID A0A0G9FRD2
Locus tag KYC43_RS19325 Protein ID WP_001350372.1
Coordinates 3942173..3942460 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag KYC43_RS19320 Protein ID WP_000063156.1
Coordinates 3941884..3942186 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYC43_RS19300 (3937698) 3937698..3938210 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
KYC43_RS19305 (3938480) 3938480..3940630 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
KYC43_RS19310 (3940680) 3940680..3940883 + 204 WP_000467859.1 YbdD/YjiX family protein -
KYC43_RS19315 (3940894) 3940894..3941850 + 957 WP_001297640.1 GTPase -
KYC43_RS19320 (3941884) 3941884..3942186 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
KYC43_RS19325 (3942173) 3942173..3942460 - 288 WP_001350372.1 BrnT family toxin Toxin
KYC43_RS19330 (3942657) 3942657..3943823 + 1167 WP_000800829.1 restriction endonuclease -
KYC43_RS19335 (3943887) 3943887..3945506 + 1620 WP_181666839.1 class I SAM-dependent DNA methyltransferase -
KYC43_RS19340 (3945496) 3945496..3946842 + 1347 WP_182212044.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11209.68 Da        Isoelectric Point: 7.4020

>T210298 WP_001350372.1 NZ_CP079884:c3942460-3942173 [Escherichia fergusonii]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T210298 NZ_CP104491:841639-842052 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGTCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
GCATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT210298 WP_000063156.1 NZ_CP079884:c3942186-3941884 [Escherichia fergusonii]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT210298 NZ_CP104491:841392-841658 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTAATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0G9FRD2

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References