Detailed information of TA system TA021027

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 30895..31566
• Replicon: plasmid pYJ016
• Accession: NC_005128
• Organism: Vibrio vulnificus YJ016

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: BJE04_RS00140
• Protein ID: WP_011149008.1
• Coordinates: 31267..31566 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: Q7MBL1
• Locus tag: BJE04_RS00135
• Protein ID: WP_011149007.1
• Coordinates: 30895..31191 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BJE04_RS00120	27492..27851	-	360	WP_011149002.1	hypothetical protein	-
BJE04_RS00125	28409..28855	-	447	WP_011149004.1	lytic transglycosylase domain-containing protein	-
BJE04_RS00130	28937..30319	-	1383	WP_011149005.1	DUF3560 domain-containing protein	-
BJE04_RS00135	30895..31191	-	297	WP_011149007.1	putative addiction module antidote protein	Antitoxin
BJE04_RS00140	31267..31566	-	300	WP_011149008.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BJE04_RS00145	31604..31822	-	219	WP_011149010.1	hypothetical protein	-
BJE04_RS25110	31867..32019	-	153	WP_167524319.1	hypothetical protein	-
BJE04_RS00150	32038..32361	-	324	WP_043876998.1	hypothetical protein	-
BJE04_RS00155	32428..32736	-	309	WP_011149014.1	DUF1738 domain-containing protein	-
BJE04_RS00160	32739..33242	-	504	WP_011149015.1	hypothetical protein	-
BJE04_RS00165	33795..34052	-	258	WP_011149016.1	hypothetical protein	-
BJE04_RS00170	34091..34399	-	309	WP_011149017.1	hypothetical protein	-
BJE04_RS00180	34978..35274	-	297	WP_011149018.1	hypothetical protein	-
BJE04_RS00185	35383..35721	-	339	WP_011149019.1	hypothetical protein	-
BJE04_RS00190	35741..36220	-	480	WP_011149021.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48508	48508

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (48-92)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11134.96 Da
• Isoelectric Point: 10.6229

>T21027 WP_011149008.1 NC_005128:c31566-31267 [Vibrio vulnificus YJ016]
MIYNIKRTEIFNDWLRELKDPQARGSIAARVQRLTQGLNGDVKPVGAGISELRIHVGKGYRVYFKRSGKQIIVLLCGGNK
KTQNQDIELAKQLAQELGL

Nucleotide

• Length: 300 bp

>T21027 NC_005128:c31566-31267 [Vibrio vulnificus YJ016]
ATGATATATAATATCAAGCGCACCGAAATCTTTAATGATTGGCTGAGGGAACTAAAAGACCCTCAAGCGCGAGGTTCAAT
CGCCGCGCGAGTGCAACGACTTACCCAAGGATTGAACGGAGACGTGAAACCTGTTGGTGCTGGTATCAGTGAACTCAGGA
TACATGTCGGAAAGGGTTACAGGGTTTACTTTAAGCGAAGCGGCAAGCAGATCATTGTTTTGTTGTGTGGCGGTAACAAG
AAAACTCAAAACCAAGACATTGAATTAGCAAAGCAGCTTGCTCAAGAACTTGGACTGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10437.90 Da
• Isoelectric Point: 4.6855

>AT21027 WP_011149007.1 NC_005128:c31191-30895 [Vibrio vulnificus YJ016]
MSKNTAMLADFDPAEYLDTPEAIQAYLDEAVASGDAAFISQSLGVIARAKGMTELAEQTGLSREALYRTLSQKGNPNFKG
FMAIISALGISLKLNVHA

Nucleotide

• Length: 297 bp

>AT21027 NC_005128:c31191-30895 [Vibrio vulnificus YJ016]
ATGTCTAAAAATACAGCTATGCTCGCAGATTTTGACCCTGCTGAGTATCTTGATACCCCAGAAGCTATACAGGCGTATCT
CGATGAAGCTGTAGCCTCTGGTGATGCAGCTTTTATCAGTCAATCTCTTGGTGTGATTGCTCGCGCTAAGGGTATGACAG
AACTTGCAGAACAAACAGGATTATCCCGAGAAGCGCTATATAGAACGCTTAGCCAAAAGGGAAACCCTAATTTTAAAGGA
TTTATGGCCATCATTTCTGCTTTGGGCATCTCTTTGAAATTGAACGTTCATGCTTAA

Structures

Antitoxin

• AlphaFold DB: Q7MBL1