Detailed information of TA system TA210245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 928966..929591
• Replicon: chromosome
• Accession: NZ_CP079855
• Organism: Agrobacterium fabrum strain LBA645

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q7D0B1
• Locus tag: KXJ62_RS04640
• Protein ID: WP_006311244.1
• Coordinates: 929232..929591 (+)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q7D0B2
• Locus tag: KXJ62_RS04635
• Protein ID: WP_010971270.1
• Coordinates: 928966..929232 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KXJ62_RS04605 (KXJ62_04600)	924140..924475	-	336	WP_010971265.1	type II toxin-antitoxin system YafQ family toxin	-
KXJ62_RS04610 (KXJ62_04605)	924472..924747	-	276	WP_010971266.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
KXJ62_RS04615 (KXJ62_04610)	924855..925463	+	609	WP_010971267.1	class I SAM-dependent methyltransferase	-
KXJ62_RS04620 (KXJ62_04615)	925639..928224	+	2586	WP_035256421.1	heavy metal translocating P-type ATPase	-
KXJ62_RS04625 (KXJ62_04620)	928221..928643	+	423	WP_006311241.1	Cu(I)-responsive transcriptional regulator	-
KXJ62_RS04630 (KXJ62_04625)	928696..928896	+	201	WP_006311242.1	heavy-metal-associated domain-containing protein	-
KXJ62_RS04635 (KXJ62_04630)	928966..929232	+	267	WP_010971270.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KXJ62_RS04640 (KXJ62_04635)	929232..929591	+	360	WP_006311244.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
KXJ62_RS04645 (KXJ62_04640)	929599..929847	-	249	WP_006311245.1	hypothetical protein	-
KXJ62_RS04650 (KXJ62_04645)	930026..931414	+	1389	WP_169539085.1	MFS transporter	-
KXJ62_RS04655 (KXJ62_04650)	931493..931666	-	174	WP_010971273.1	hypothetical protein	-
KXJ62_RS04660 (KXJ62_04655)	931735..933453	-	1719	WP_035256424.1	glycoside hydrolase family 32 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-111)

Antitoxin

• SpoVT-AbrB domain (14-52)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 12857.04 Da
• Isoelectric Point: 8.2782

>T210245 WP_006311244.1 NZ_CP079855:929232-929591 [Agrobacterium fabrum]
MVRNQIPKRGDVYLVDLNPVVGSEIKDEHRCVVITPREINAVGLCLVVPVTTGGMFTRKAGLAVNISGHKTTGVALCNQV
RSMDIVARVAQKKAKYIETLDDATIDEIAGRVISMIDPA

Nucleotide

• Length: 360 bp

>T210245 NZ_CP104479:871123-871536 [Salmonella enterica]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCATGGCGCGCTCAGTGGATCTCATTGCTCATTCATGGGCTGGTTGCCGC
GGTGATTTTATTGATGCCCTGGCCGCTGAGTTATACCCCGTTATGGATGATACTACTGTCGCTGGTCGTGTTTGACTGTG
TACGTAGCCAGCGCAGGATTAATACCTGCCAGGGAGAGATCAAGCTACTCATGGACGGGCGCTTACGCTGGCAGGGGCAG
GACTGGACGCTCTTGCGTCCGCCCTGGTTACTGAAGAGCGGGATGGTGTTGCGTTTACGCGCAGAGTCTGGACGTCATCA
ACATTTATGGCTGGCGGCGGATAGCATGGAAGAAGCGGAATGGCGTGAGTTGCGTCGGATACTGTTACAGCAGCCGATAT
CAGGCCAACACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9423.02 Da
• Isoelectric Point: 5.1106

>AT210245 WP_010971270.1 NZ_CP079855:928966-929232 [Agrobacterium fabrum]
MTVTTKIRRQGGAAVMTIPPALLKMLGLEIGEQLTLEVDNGALVASPVRLEKKRFTLAELLDGAEEVAALNARERAWDTA
PPVGKEAL

Nucleotide

• Length: 267 bp

>AT210245 NZ_CP104479:870876-871142 [Salmonella enterica]
ATGGATATTCACAATAAAGCACGTATTCACTGGGCATGTCGTCGCGGTATGCGCGAACTTGATATTTCCATCATGCCGTT
TTTTGAACATGAGTACGATAGTTTAAGTGATGAAGAAAAGCGTATTTTTGTCCGTTTGTTGCAGAGTGATGACCCGGATT
TATTTAACTGGTTGATGAATCACGGTAAACCGGCTGACGCTGAACTGGAACAGATGGTCCGACTCATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: Q7D0B1

Antitoxin

• AlphaFold DB: Q7D0B2