Detailed information of TA system TA021020

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 5020966..5021409
• Replicon: chromosome
• Accession: NC_005126
• Organism: Photorhabdus luminescens subsp. laumondii TTO1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: PLU_RS25735
• Protein ID: WP_011148391.1
• Coordinates: 5021185..5021409 (+)
• Length: 75 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q7MZJ1
• Locus tag: PLU_RS21230
• Protein ID: WP_011148390.1
• Coordinates: 5020966..5021184 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PLU_RS21210 (plu4290)	5016724..5017713	-	990	WP_011148385.1	glycosyltransferase	-
PLU_RS21215 (plu4291)	5018093..5019289	+	1197	WP_011148386.1	aspartyl protease family protein	-
PLU_RS21220 (plu4292)	5019459..5020070	-	612	WP_011148387.1	hypothetical protein	-
PLU_RS25730 (plu4293)	5020204..5020440	-	237	WP_011148388.1	helix-turn-helix domain-containing protein	-
PLU_RS21225 (plu4294)	5020590..5020799	+	210	WP_011148389.1	helix-turn-helix domain-containing protein	-
PLU_RS21230 (plu4295)	5020966..5021184	+	219	WP_011148390.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
PLU_RS25735 (plu4296)	5021185..5021409	+	225	WP_011148391.1	CcdB family protein	Toxin
PLU_RS21235 (plu4297)	5021355..5022344	-	990	WP_011148392.1	AraC family transcriptional regulator	-
PLU_RS21240 (plu4298)	5022459..5023100	+	642	WP_011148393.1	HD domain-containing protein	-
PLU_RS21245 (plu4299)	5023336..5023572	-	237	WP_011148394.1	helix-turn-helix domain-containing protein	-
PLU_RS21250 (plu4300)	5023924..5024589	-	666	WP_109792001.1	GntR family transcriptional regulator	-
PLU_RS21255 (plu4301)	5025156..5025884	+	729	WP_011148396.1	DUF969 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-54)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 75 a.a.
• Molecular weight: 8681.12 Da
• Isoelectric Point: 8.2844

>T21020 WP_011148391.1 NC_005126:5021185-5021409 [Photorhabdus laumondii subsp. laumondii TTO1]
MQFVVYQYKRSESKYSMFVDVQRDIIETPERRMAIPLVEAHHFSSKVSRHLFPTSGSQFSSFVPLCCVCMNIFL

Nucleotide

• Length: 225 bp

>T21020 NC_005126:5021185-5021409 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGCAATTTGTTGTTTATCAATACAAACGGAGCGAAAGCAAATACAGTATGTTTGTTGATGTACAACGTGACATCATTGA
AACACCAGAAAGGCGTATGGCTATTCCTTTAGTTGAAGCGCATCATTTTTCAAGCAAGGTGAGTCGTCATCTATTTCCCA
CGTCTGGCTCTCAGTTTTCTTCATTTGTGCCGCTCTGCTGCGTCTGTATGAATATTTTTCTGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8379.39 Da
• Isoelectric Point: 8.5552

>AT21020 WP_011148390.1 NC_005126:5020966-5021184 [Photorhabdus laumondii subsp. laumondii TTO1]
MKHRVNVTFDKENYLILNSAGVNISGFVNKAMTREAQRIKAESWKADNRKGLEEIAQFIEQYGSFADENRNW

Nucleotide

• Length: 219 bp

>AT21020 NC_005126:5020966-5021184 [Photorhabdus laumondii subsp. laumondii TTO1]
ATGAAACATAGAGTTAACGTAACTTTTGATAAAGAAAATTATCTGATCCTGAATTCAGCCGGTGTAAATATTTCAGGTTT
TGTTAATAAAGCCATGACCAGAGAAGCTCAACGAATCAAAGCTGAAAGTTGGAAAGCAGATAATCGCAAAGGATTGGAAG
AAATTGCTCAATTTATTGAACAATATGGCTCATTTGCTGATGAAAACAGGAACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6L9JTU9