Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 981209..981795 Replicon chromosome
Accession NZ_CP079827
Organism Pseudomonas sp. HD6515

Toxin (Protein)


Gene name PumA Uniprot ID A0A2I0CCV1
Locus tag KW568_RS04560 Protein ID WP_004576348.1
Coordinates 981209..981502 (+) Length 98 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID I3UY28
Locus tag KW568_RS04565 Protein ID WP_004576347.1
Coordinates 981499..981795 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KW568_RS04535 (KW568_04510) 976810..977664 + 855 WP_003248578.1 putative selenate ABC transporter substrate-binding protein -
KW568_RS04540 (KW568_04515) 977661..978470 + 810 WP_004576351.1 phosphonate ABC transporter ATP-binding protein -
KW568_RS04545 (KW568_04520) 978464..979291 + 828 WP_004576350.1 ABC transporter permease subunit -
KW568_RS04550 (KW568_04525) 979288..980055 + 768 WP_004576349.1 phosphonate ABC transporter, permease protein PhnE -
KW568_RS04555 (KW568_04530) 980092..980985 - 894 WP_003248571.1 bestrophin family ion channel -
KW568_RS04560 (KW568_04535) 981209..981502 + 294 WP_004576348.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KW568_RS04565 (KW568_04540) 981499..981795 + 297 WP_004576347.1 putative addiction module antidote protein Antitoxin
KW568_RS04570 (KW568_04545) 981806..982285 - 480 WP_004576346.1 GNAT family N-acetyltransferase -
KW568_RS04575 (KW568_04550) 982456..983103 - 648 WP_033726680.1 serine protease -
KW568_RS04585 (KW568_04560) 983556..984605 + 1050 WP_004576301.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
KW568_RS04590 (KW568_04565) 984620..985735 + 1116 WP_003248558.1 tRNA guanosine(34) transglycosylase Tgt -
KW568_RS04595 (KW568_04570) 985778..986113 + 336 WP_003248556.1 preprotein translocase subunit YajC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(2-96)

Antitoxin

Uncharacterized protein HI1420

(4-93)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 10751.55 Da        Isoelectric Point: 10.8302

>T210167 WP_004576348.1 NZ_CP079827:981209-981502 [Pseudomonas sp. HD6515]
MFLVEQTESFAAWLSSLRDLRARLAIGRRLERAAAGNLGDFKWLGGGIGELRIDVAAGYRVYFTQKGQRLVLLLVGGDKS
TQAADILNARKLLKEMK

Download         Length: 294 bp

>T210167 NZ_CP104442:4860720-4861031 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 10676.33 Da        Isoelectric Point: 6.7249

>AT210167 WP_004576347.1 NZ_CP079827:981499-981795 [Pseudomonas sp. HD6515]
MSEKLIPFDMAALLSSDEAISEYLSQVLSNGDTEEIIRALGHIARARGMTHIATESGLGRESLYKALSPGAKPRFDTVLR
VIRALGVDLLVQPHAMTR

Download         Length: 297 bp

>AT210167 NZ_CP104442:4861032-4861322 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I0CCV1

Antitoxin

Source ID Structure
AlphaFold DB A0A2I0CD26

References