Detailed information of TA system TA210167

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 981209..981795
• Replicon: chromosome
• Accession: NZ_CP079827
• Organism: Pseudomonas sp. HD6515

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A2I0CCV1
• Locus tag: KW568_RS04560
• Protein ID: WP_004576348.1
• Coordinates: 981209..981502 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: I3UY28
• Locus tag: KW568_RS04565
• Protein ID: WP_004576347.1
• Coordinates: 981499..981795 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW568_RS04535 (KW568_04510)	976810..977664	+	855	WP_003248578.1	putative selenate ABC transporter substrate-binding protein	-
KW568_RS04540 (KW568_04515)	977661..978470	+	810	WP_004576351.1	phosphonate ABC transporter ATP-binding protein	-
KW568_RS04545 (KW568_04520)	978464..979291	+	828	WP_004576350.1	ABC transporter permease subunit	-
KW568_RS04550 (KW568_04525)	979288..980055	+	768	WP_004576349.1	phosphonate ABC transporter, permease protein PhnE	-
KW568_RS04555 (KW568_04530)	980092..980985	-	894	WP_003248571.1	bestrophin family ion channel	-
KW568_RS04560 (KW568_04535)	981209..981502	+	294	WP_004576348.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KW568_RS04565 (KW568_04540)	981499..981795	+	297	WP_004576347.1	putative addiction module antidote protein	Antitoxin
KW568_RS04570 (KW568_04545)	981806..982285	-	480	WP_004576346.1	GNAT family N-acetyltransferase	-
KW568_RS04575 (KW568_04550)	982456..983103	-	648	WP_033726680.1	serine protease	-
KW568_RS04585 (KW568_04560)	983556..984605	+	1050	WP_004576301.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
KW568_RS04590 (KW568_04565)	984620..985735	+	1116	WP_003248558.1	tRNA guanosine(34) transglycosylase Tgt	-
KW568_RS04595 (KW568_04570)	985778..986113	+	336	WP_003248556.1	preprotein translocase subunit YajC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-96)

Antitoxin

• Uncharacterized protein HI1420 (4-93)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10751.55 Da
• Isoelectric Point: 10.8302

>T210167 WP_004576348.1 NZ_CP079827:981209-981502 [Pseudomonas sp. HD6515]
MFLVEQTESFAAWLSSLRDLRARLAIGRRLERAAAGNLGDFKWLGGGIGELRIDVAAGYRVYFTQKGQRLVLLLVGGDKS
TQAADILNARKLLKEMK

Nucleotide

• Length: 294 bp

>T210167 NZ_CP104442:4860720-4861031 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10676.33 Da
• Isoelectric Point: 6.7249

>AT210167 WP_004576347.1 NZ_CP079827:981499-981795 [Pseudomonas sp. HD6515]
MSEKLIPFDMAALLSSDEAISEYLSQVLSNGDTEEIIRALGHIARARGMTHIATESGLGRESLYKALSPGAKPRFDTVLR
VIRALGVDLLVQPHAMTR

Nucleotide

• Length: 297 bp

>AT210167 NZ_CP104442:4861032-4861322 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A2I0CCV1

Antitoxin

• AlphaFold DB: A0A2I0CD26