Detailed information of TA system TA210165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 14658..15117
• Replicon: plasmid pHD6454-KPC
• Accession: NZ_CP079826
• Organism: Aeromonas veronii strain HD6454

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: KW556_RS23870
• Protein ID: WP_259589269.1
• Coordinates: 14866..15117 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: KW556_RS23865
• Protein ID: WP_259589267.1
• Coordinates: 14658..14882 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW556_RS23830 (KW556_23725)	9669..10262	-	594	Protein_12	Tn3 family transposase	-
KW556_RS23835 (KW556_23730)	10274..10783	-	510	Protein_13	TetR/AcrR family transcriptional regulator	-
KW556_RS23840	11105..11170	+	66	WP_259589285.1	hypothetical protein	-
KW556_RS23845 (KW556_23740)	11347..11589	-	243	WP_134997961.1	plasmid replication protein RepB	-
KW556_RS23850 (KW556_23745)	11695..12195	-	501	WP_134997962.1	hypothetical protein	-
KW556_RS23855 (KW556_23750)	12322..13176	-	855	WP_236544954.1	site-specific integrase	-
KW556_RS23860 (KW556_23755)	13205..13627	-	423	WP_201035626.1	hypothetical protein	-
KW556_RS23865 (KW556_23760)	14658..14882	+	225	WP_259589267.1	ribbon-helix-helix protein, CopG family	Antitoxin
KW556_RS23870 (KW556_23765)	14866..15117	+	252	WP_259589269.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KW556_RS23875 (KW556_23770)	15850..16545	+	696	WP_259589271.1	hypothetical protein	-
KW556_RS23885 (KW556_23780)	18449..18718	+	270	WP_259589274.1	hypothetical protein	-
KW556_RS23890 (KW556_23785)	18788..19039	+	252	WP_259589275.1	DUF4160 domain-containing protein	-
KW556_RS23895 (KW556_23790)	19023..19445	+	423	WP_259589277.1	DUF2442 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2	-	1..51662	51662

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-77)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-38)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9344.18 Da
• Isoelectric Point: 10.2724

>T210165 WP_259589269.1 NZ_CP079826:14866-15117 [Aeromonas veronii]
MAWKVELTDTAAKQLASLDKAQAQRITKSLRRLMLLEDPKCVGKTLTGNMRICLRYRTGDSRLIYELKNNCLIVVAVMVS
GCK

Nucleotide

• Length: 252 bp

>T210165 NZ_CP104442:c4372117-4371887 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8718.81 Da
• Isoelectric Point: 4.4442

>AT210165 WP_259589267.1 NZ_CP079826:14658-14882 [Aeromonas veronii]
MLAIRLPSDIEDRLAHLAKETGRTKTYYAREAILTYLEDLEDYYLADRVMERVRKGEEHLFSLDDVERDLGLES

Nucleotide

• Length: 225 bp

>AT210165 NZ_CP104442:c4371890-4371531 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA