Detailed information of TA system TA210162

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 4492619..4494333
• Replicon: chromosome
• Accession: NZ_CP079823
• Organism: Aeromonas veronii strain HD6454

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: KW556_RS21090
• Protein ID: WP_058396005.1
• Coordinates: 4493680..4494333 (-)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: KW556_RS21085
• Protein ID: WP_259584242.1
• Coordinates: 4492619..4493683 (-)
• Length: 355 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KW556_RS21070 (KW556_20990)	4487777..4488547	+	771	Protein_4082	restriction endonuclease	-
KW556_RS21075 (KW556_20995)	4488700..4489413	-	714	WP_005331303.1	SprT family zinc-dependent metalloprotease	-
KW556_RS21080 (KW556_21000)	4489413..4492544	-	3132	WP_259584241.1	HsdR family type I site-specific deoxyribonuclease	-
KW556_RS21085 (KW556_21005)	4492619..4493683	-	1065	WP_259584242.1	macro domain-containing protein	Antitoxin
KW556_RS21090 (KW556_21010)	4493680..4494333	-	654	WP_058396005.1	DUF4433 domain-containing protein	Toxin
KW556_RS21095 (KW556_21015)	4494598..4495962	-	1365	WP_259584246.1	restriction endonuclease subunit S	-
KW556_RS21100 (KW556_21020)	4495962..4498409	-	2448	WP_259584247.1	type I restriction-modification system subunit M	-
KW556_RS21105 (KW556_21025)	4498595..4499041	-	447	WP_043852095.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4483901..4532305	48404

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-216)

Antitoxin

• Macro domain (18-131)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 25357.97 Da
• Isoelectric Point: 7.0172

>T210162 WP_058396005.1 NZ_CP079823:c4494333-4493680 [Aeromonas veronii]
MAYDYSQSLNPQKALIFRIVHRDNLEWILTHGLWAGNGQQQNPHWVHIGLPELIAKRARHPVPLVPYGVLNDYVPFYFTP
FSPMLSNIHSGRGVPQRDNDEILILVSSLHRMAELGLPFVFTDSHAYYQWANFYVDLGDLGRIDWPLLQRRDFKRDADDL
SKLERYQAEALIHQHCPIEALLGVVCYNEEIKNRVEQCMNRVGVARPVYVRDGWYFR

Nucleotide

• Length: 654 bp

>T210162 NZ_CP104442:c3780650-3780243 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 355 a.a.
• Molecular weight: 39616.76 Da
• Isoelectric Point: 6.5002

>AT210162 WP_259584242.1 NZ_CP079823:c4493683-4492619 [Aeromonas veronii]
MIRYTTGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPANMRAYAEACKQGDVVTGKMFITQTGELLGPRWIVNFPTK
QHWRNPAQMAWIQDGLQDLRAWLQAQQVSSIAIPPLGAGNGGLPWPEVKQAIETALGDLDIDILLYEPSSQYQNVAKRGG
VAELTPARALIAELVRRYWFLGMECSLLEIQKLAWFLARVIGRNPLGDPLQLQFKAHRYGPYANNLTHLLNAMDGSYLQS
DKRIPDSKPFDVIWFNEAKKQELAVYLQTEAKAFLPILEQTTALIDGFESPYGMELLATVDWLIAEEGCAPTLSVLREGL
HRWPAGTPWGERKARLFDDRSLQIALTRLQQGLV

Nucleotide

• Length: 1065 bp

>AT210162 NZ_CP104442:c3780897-3780631 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA