Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 36980..37505 Replicon plasmid pS465MC_p2
Accession NZ_CP079809
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC02_S465MC

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag KWL80_RS28045 Protein ID WP_001159868.1
Coordinates 37200..37505 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag KWL80_RS28040 Protein ID WP_000813634.1
Coordinates 36980..37198 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWL80_RS28020 (KWL80_27995) 32918..34195 - 1278 WP_016338369.1 HlyD family secretion protein -
KWL80_RS28550 34854..35390 + 537 WP_223825639.1 hypothetical protein -
KWL80_RS28555 35408..35731 + 324 WP_085842301.1 hypothetical protein -
KWL80_RS28030 (KWL80_28005) 35772..36188 - 417 WP_048983057.1 type II toxin-antitoxin system VapC family toxin -
KWL80_RS28035 (KWL80_28010) 36185..36415 - 231 WP_016338373.1 type II toxin-antitoxin system VapB family antitoxin -
KWL80_RS28040 (KWL80_28015) 36980..37198 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KWL80_RS28045 (KWL80_28020) 37200..37505 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
KWL80_RS28050 (KWL80_28025) 37506..38312 + 807 WP_000016982.1 site-specific integrase -
KWL80_RS28055 (KWL80_28030) 39086..39841 + 756 WP_000852146.1 replication initiation protein RepE -
KWL80_RS28060 (KWL80_28035) 40429..41595 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrB1 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaTEM-1B / tet(D) - 1..55140 55140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T210154 WP_001159868.1 NZ_CP079809:37200-37505 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T210154 NZ_CP104442:2198783-2198959 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT210154 WP_000813634.1 NZ_CP079809:36980-37198 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT210154 NZ_CP104442:2199005-2199421 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References