Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 35772..36415 Replicon plasmid pS465MC_p2
Accession NZ_CP079809
Organism Klebsiella pneumoniae subsp. pneumoniae strain WRC02_S465MC

Toxin (Protein)


Gene name vagD Uniprot ID A0A5Q9LNG2
Locus tag KWL80_RS28030 Protein ID WP_048983057.1
Coordinates 35772..36188 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID R4IT22
Locus tag KWL80_RS28035 Protein ID WP_016338373.1
Coordinates 36185..36415 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KWL80_RS28015 (KWL80_27990) 30820..32928 - 2109 WP_016338368.1 peptidase domain-containing ABC transporter -
KWL80_RS28020 (KWL80_27995) 32918..34195 - 1278 WP_016338369.1 HlyD family secretion protein -
KWL80_RS28550 34854..35390 + 537 WP_223825639.1 hypothetical protein -
KWL80_RS28555 35408..35731 + 324 WP_085842301.1 hypothetical protein -
KWL80_RS28030 (KWL80_28005) 35772..36188 - 417 WP_048983057.1 type II toxin-antitoxin system VapC family toxin Toxin
KWL80_RS28035 (KWL80_28010) 36185..36415 - 231 WP_016338373.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KWL80_RS28040 (KWL80_28015) 36980..37198 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
KWL80_RS28045 (KWL80_28020) 37200..37505 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
KWL80_RS28050 (KWL80_28025) 37506..38312 + 807 WP_000016982.1 site-specific integrase -
KWL80_RS28055 (KWL80_28030) 39086..39841 + 756 WP_000852146.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid qnrB1 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaTEM-1B / tet(D) - 1..55140 55140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15022.52 Da        Isoelectric Point: 9.2957

>T210153 WP_048983057.1 NZ_CP079809:c36188-35772 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFARVPGLVLEDWVK

Download         Length: 417 bp

>T210153 NZ_CP104442:c2084287-2084093 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGGTATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8751.77 Da        Isoelectric Point: 4.3533

>AT210153 WP_016338373.1 NZ_CP079809:c36415-36185 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWSSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT210153 NZ_CP104442:2083916-2084094 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAATTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LNG2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q9LN61

References