Detailed information of TA system TA210134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 23999..24524
• Replicon: plasmid unnamed1
• Accession: NZ_CP079803
• Organism: Klebsiella variicola strain EMBL-1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: KU883_RS27385
• Protein ID: WP_012539978.1
• Coordinates: 24219..24524 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: R4WC25
• Locus tag: KU883_RS27380
• Protein ID: WP_015632547.1
• Coordinates: 23999..24217 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KU883_RS27365 (KU883_27350)	19526..19780	-	255	WP_032700773.1	hypothetical protein	-
KU883_RS27370 (KU883_27355)	20060..22309	-	2250	WP_077138867.1	P-loop NTPase fold protein	-
KU883_RS27375 (KU883_27360)	22311..23405	-	1095	WP_077138868.1	transcriptional regulator	-
KU883_RS27380 (KU883_27365)	23999..24217	+	219	WP_015632547.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KU883_RS27385 (KU883_27370)	24219..24524	+	306	WP_012539978.1	type II toxin-antitoxin system toxin CcdB	Toxin
KU883_RS27390 (KU883_27375)	24711..25715	+	1005	WP_077138869.1	hypothetical protein	-
KU883_RS27395 (KU883_27380)	25913..26692	+	780	WP_029497485.1	site-specific integrase	-
KU883_RS27400 (KU883_27385)	26750..27007	-	258	WP_032433931.1	hypothetical protein	-
KU883_RS27405 (KU883_27390)	27135..27248	-	114	WP_032072094.1	hypothetical protein	-
KU883_RS27410 (KU883_27395)	27880..28635	+	756	WP_012539983.1	replication initiation protein RepE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..98922	98922

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11601.27 Da
• Isoelectric Point: 6.4661

>T210134 WP_012539978.1 NZ_CP079803:24219-24524 [Klebsiella variicola]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T210134 NZ_CP104405:3636967-3637266 [Enterobacter hormaechei subsp. steigerwaltii]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATCCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8362.31 Da
• Isoelectric Point: 4.7247

>AT210134 WP_015632547.1 NZ_CP079803:23999-24217 [Klebsiella variicola]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT210134 NZ_CP104405:3637277-3637642 [Enterobacter hormaechei subsp. steigerwaltii]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCATTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2L1KT86