Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5584925..5585550 Replicon chromosome
Accession NZ_CP079802
Organism Klebsiella variicola strain EMBL-1

Toxin (Protein)


Gene name vapC Uniprot ID A0A1F2M041
Locus tag KU883_RS26895 Protein ID WP_008807903.1
Coordinates 5584925..5585308 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KU883_RS26900 Protein ID WP_004150355.1
Coordinates 5585308..5585550 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU883_RS26880 (KU883_26865) 5582291..5583193 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KU883_RS26885 (KU883_26870) 5583190..5583825 + 636 WP_008807902.1 formate dehydrogenase cytochrome b556 subunit -
KU883_RS26890 (KU883_26875) 5583822..5584751 + 930 WP_055323209.1 formate dehydrogenase accessory protein FdhE -
KU883_RS26895 (KU883_26880) 5584925..5585308 - 384 WP_008807903.1 type II toxin-antitoxin system VapC family toxin Toxin
KU883_RS26900 (KU883_26885) 5585308..5585550 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KU883_RS26905 (KU883_26890) 5585755..5586672 + 918 WP_032733990.1 alpha/beta hydrolase -
KU883_RS26910 (KU883_26895) 5586687..5587628 - 942 WP_012543287.1 fatty acid biosynthesis protein FabY -
KU883_RS26915 (KU883_26900) 5587673..5588110 - 438 WP_012543288.1 D-aminoacyl-tRNA deacylase -
KU883_RS26920 (KU883_26905) 5588107..5588967 - 861 WP_008807907.1 virulence factor BrkB family protein -
KU883_RS26925 (KU883_26910) 5588961..5589560 - 600 WP_008807908.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T210133 WP_008807903.1 NZ_CP079802:c5585308-5584925 [Klebsiella variicola]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYELHPE

Download         Length: 384 bp

>T210133 NZ_CP104405:c2957886-2957533 [Enterobacter hormaechei subsp. steigerwaltii]
GTGTGGGCGATTAAAACTACGGACAGGTTTGACGACTGGTTCACCTCATTAAACGATAGCGAGAGAGCCAGCGTACTGGC
CGCGCTACTTGTTTTGCGGGAGAGGGGACCGGGTCTGTCCAGACCGTATGCCGATACGCTGAAAGGCTCCCGCCACAGCA
ACATGAAAGAGTTGCGTATCCAGAGTAAGGGTGATCCGCTGAGGGCATTTTTCGCTTTCGATCCGAACCGTACGGGTATT
GTACTGTGTGCGGGCAATAAGGTCGGTAATGAAAGGCGATTTTACGATGAGATGCTCCTCGTTGCGGATCGGGAATACAC
TCGCTGGCTGAACACATTAAAAGAGAGGAATTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT210133 WP_004150355.1 NZ_CP079802:c5585550-5585308 [Klebsiella variicola]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT210133 NZ_CP104405:c2957528-2957226 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGGCAGAACCCTGGAGCAGCTTATTGCAGATGAAAAACCTGAGGTTGTTGCCAGCGCACAGGCAATGGCAACGGATAT
TTTGCTCAATATCCATCTGGCCGAGTTACGTGAAAAAGTGCAAAAAACGCAGGTAGATATGGCCAGGGCGTTGGGTATTA
AACAACCTACTGTCGCCGTCATGGAAAAGGCAGGACGCGATATTAAGCTTTCTTCTTTGAAACGCTACGTTGAGGCCGCC
GGAGGGAAGCTACGGCTGGATGTAGAACTACCCGATGGTTCTCATTACGAGTTTGTGTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2M041

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References