Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4372337..4372956 Replicon chromosome
Accession NZ_CP079802
Organism Klebsiella variicola strain EMBL-1

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KU883_RS21175 Protein ID WP_002892050.1
Coordinates 4372738..4372956 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A1F2MBN7
Locus tag KU883_RS21170 Protein ID WP_008805436.1
Coordinates 4372337..4372711 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU883_RS21160 (KU883_21150) 4367492..4368685 + 1194 WP_012542667.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KU883_RS21165 (KU883_21155) 4368708..4371854 + 3147 WP_008805437.1 multidrug efflux RND transporter permease subunit AcrB -
KU883_RS21170 (KU883_21160) 4372337..4372711 + 375 WP_008805436.1 Hha toxicity modulator TomB Antitoxin
KU883_RS21175 (KU883_21165) 4372738..4372956 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KU883_RS21180 (KU883_21170) 4373115..4373681 + 567 WP_032438710.1 maltose O-acetyltransferase -
KU883_RS21185 (KU883_21175) 4373653..4373781 - 129 Protein_4172 hypothetical protein -
KU883_RS21190 (KU883_21180) 4373818..4374288 + 471 WP_008805434.1 YlaC family protein -
KU883_RS21195 (KU883_21185) 4374257..4375714 - 1458 WP_016160392.1 PLP-dependent aminotransferase family protein -
KU883_RS21200 (KU883_21190) 4375815..4376513 + 699 WP_012968815.1 GNAT family protein -
KU883_RS21205 (KU883_21195) 4376510..4376650 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KU883_RS21210 (KU883_21200) 4376650..4376913 - 264 WP_008805431.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T210131 WP_002892050.1 NZ_CP079802:4372738-4372956 [Klebsiella variicola]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T210131 NZ_CP104405:c2393534-2393049 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATACCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATTATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTCAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14384.06 Da        Isoelectric Point: 4.8989

>AT210131 WP_008805436.1 NZ_CP079802:4372337-4372711 [Klebsiella variicola]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYTEDNKLVAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT210131 NZ_CP104405:c2393788-2393522 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2MBN7

References