Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | hicAB/UPF0150(antitoxin) |
| Location | 3503592..3504223 | Replicon | chromosome |
| Accession | NZ_CP079802 | ||
| Organism | Klebsiella variicola strain EMBL-1 | ||
Toxin (Protein)
| Gene name | hicA | Uniprot ID | A0A1Q8YTV3 |
| Locus tag | KU883_RS17060 | Protein ID | WP_012542177.1 |
| Coordinates | 3504047..3504223 (-) | Length | 59 a.a. |
Antitoxin (Protein)
| Gene name | hicB | Uniprot ID | A0A2X1QFN9 |
| Locus tag | KU883_RS17055 | Protein ID | WP_012542176.1 |
| Coordinates | 3503592..3503999 (-) | Length | 136 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KU883_RS17010 (KU883_17000) | 3498724..3499155 | - | 432 | WP_077269189.1 | hypothetical protein | - |
| KU883_RS17015 (KU883_17005) | 3499152..3499475 | - | 324 | WP_219163134.1 | hypothetical protein | - |
| KU883_RS17020 (KU883_17010) | 3499427..3499789 | - | 363 | WP_032428746.1 | HNH endonuclease | - |
| KU883_RS17025 (KU883_17015) | 3499773..3499937 | - | 165 | WP_162838183.1 | hypothetical protein | - |
| KU883_RS17030 (KU883_17020) | 3500116..3500340 | + | 225 | WP_032749552.1 | hypothetical protein | - |
| KU883_RS17035 (KU883_17025) | 3500379..3500816 | - | 438 | WP_065888617.1 | hypothetical protein | - |
| KU883_RS17040 (KU883_17030) | 3501766..3502116 | - | 351 | WP_012542173.1 | hypothetical protein | - |
| KU883_RS17045 (KU883_17035) | 3502113..3502610 | - | 498 | WP_086631574.1 | lysozyme | - |
| KU883_RS17050 (KU883_17040) | 3502588..3502857 | - | 270 | WP_023289178.1 | phage holin | - |
| KU883_RS17055 (KU883_17045) | 3503592..3503999 | - | 408 | WP_012542176.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
| KU883_RS17060 (KU883_17050) | 3504047..3504223 | - | 177 | WP_012542177.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
| KU883_RS17065 (KU883_17055) | 3504588..3505733 | + | 1146 | WP_023328101.1 | nucleoid-associated protein | - |
| KU883_RS17070 (KU883_17060) | 3505723..3506949 | + | 1227 | WP_219163136.1 | hypothetical protein | - |
| KU883_RS17075 (KU883_17065) | 3506944..3507288 | - | 345 | WP_064147746.1 | antiterminator Q family protein | - |
| KU883_RS17080 (KU883_17070) | 3507301..3508332 | - | 1032 | WP_219163138.1 | DUF968 domain-containing protein | - |
| KU883_RS17085 (KU883_17075) | 3508532..3508924 | - | 393 | WP_016528965.1 | LexA DNA binding domain-containing protein | - |
| KU883_RS17090 (KU883_17080) | 3508965..3509222 | - | 258 | WP_022065893.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | - | 3475417..3576495 | 101078 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 59 a.a. Molecular weight: 6632.83 Da Isoelectric Point: 11.0174
>T210129 WP_012542177.1 NZ_CP079802:c3504223-3504047 [Klebsiella variicola]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK
Download Length: 177 bp
>T210129 NZ_CP104405:c1241040-1240729 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCTAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTGATGTTTTTTGCTGATTTTGAGCGTGGAAAGATC
TTCATCAAACACATCAGCACTCACGCAGACTATGACAAGCTCACGGACTACTACCGGAGAACGAAAGAATGA
ATGCACATAGTCTCAAGAGCGCCTTTCGACACAGCGGCCAGACATTATCCCAATCAAGCACAAGCCCTGGACGATCTTTA
CCGGGTACTAAAAAAGGAACACTACCCCTCACCAGACGAGATGCGAAAGCGCTTTCCTAGCCTGGACCGGATGAAATACC
GGGAAAAATGGTGGGTCATTGATGTGGGTGGCGGGCATCTAAGAGTGATGTTTTTTGCTGATTTTGAGCGTGGAAAGATC
TTCATCAAACACATCAGCACTCACGCAGACTATGACAAGCTCACGGACTACTACCGGAGAACGAAAGAATGA
Antitoxin
Download Length: 136 a.a. Molecular weight: 14977.99 Da Isoelectric Point: 4.4277
>AT210129 WP_012542176.1 NZ_CP079802:c3503999-3503592 [Klebsiella variicola]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARMINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARMINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH
Download Length: 408 bp
>AT210129 NZ_CP104405:c1240732-1240313 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAGCTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCCGATAACCCGCTGGTAGATATGCTCACTG
CCAAAATCGATAAGTATGAAAACGCGTCCCCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGCATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGTGATTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTTAACGGACAGCGCACGTTAACGCTAGATCATATGCGAGCGCTGGCGAAGCGTTTCGGTTTGCCGGTGA
GCGCATTTGTGGGGAACTGA
ATGAATTATGCAGCAGCCATCAAAGCGGCAAACGCTCTGACAAACGAGCTCCCTTTCCTGGGTTCAAGCCCGTCGCGGCA
AGACTACGAGGATGCGCTGGCGCTGGTGGAGTATCTTATCGAGCACGACCCCGATAACCCGCTGGTAGATATGCTCACTG
CCAAAATCGATAAGTATGAAAACGCGTCCCCGGAGTTTGCAGAATTTAACGCGCGTATTGCCAGCATTCCGTCAGGCGTT
GCGTTACTGCGAACGCTAATGGATCAGTACCAGCTCACTCAGAGTGATTTCGAGAACGAGATTGGTAAGAAGTCTCTGGT
AAGTCGGATCCTTAACGGACAGCGCACGTTAACGCTAGATCATATGCGAGCGCTGGCGAAGCGTTTCGGTTTGCCGGTGA
GCGCATTTGTGGGGAACTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A1Q8YTV3 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2X1QFN9 |