Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1881443..1882033 Replicon chromosome
Accession NZ_CP079802
Organism Klebsiella variicola strain EMBL-1

Toxin (Protein)


Gene name pemK Uniprot ID A0A1F2LYA2
Locus tag KU883_RS09080 Protein ID WP_008804165.1
Coordinates 1881701..1882033 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A1F2LZQ3
Locus tag KU883_RS09075 Protein ID WP_012541132.1
Coordinates 1881443..1881700 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU883_RS09055 (KU883_09055) 1877052..1877627 + 576 WP_077139353.1 hypothetical protein -
KU883_RS09060 (KU883_09060) 1877804..1878640 + 837 WP_008804155.1 alpha/beta hydrolase -
KU883_RS09065 (KU883_09065) 1878846..1879817 + 972 WP_012541130.1 sensor domain-containing diguanylate cyclase -
KU883_RS09070 (KU883_09070) 1879814..1880914 - 1101 WP_048268588.1 AarF/UbiB family protein -
KU883_RS09075 (KU883_09075) 1881443..1881700 + 258 WP_012541132.1 antitoxin Antitoxin
KU883_RS09080 (KU883_09080) 1881701..1882033 + 333 WP_008804165.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
KU883_RS09085 (KU883_09085) 1882361..1883599 + 1239 WP_219163259.1 hypothetical protein -
KU883_RS09090 (KU883_09090) 1883769..1885493 - 1725 WP_219163260.1 response regulator receiver domain -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11867.71 Da        Isoelectric Point: 10.1839

>T210119 WP_008804165.1 NZ_CP079802:1881701-1882033 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Download         Length: 333 bp

>T210119 NZ_CP104403:c3869107-3868697 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGGCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGGGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAATACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9420.91 Da        Isoelectric Point: 4.4915

>AT210119 WP_012541132.1 NZ_CP079802:1881443-1881700 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT210119 NZ_CP104403:c3869354-3869088 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1F2LYA2

Antitoxin

Source ID Structure
AlphaFold DB A0A1F2LZQ3

References