Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 326536..327122 Replicon chromosome
Accession NZ_CP079802
Organism Klebsiella variicola strain EMBL-1

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag KU883_RS01495 Protein ID WP_002920800.1
Coordinates 326754..327122 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag KU883_RS01490 Protein ID WP_004174006.1
Coordinates 326536..326757 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KU883_RS01470 (KU883_01470) 322693..323619 + 927 WP_012540389.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KU883_RS01475 (KU883_01475) 323616..324893 + 1278 WP_008806971.1 branched chain amino acid ABC transporter permease LivM -
KU883_RS01480 (KU883_01480) 324890..325657 + 768 WP_008806972.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
KU883_RS01485 (KU883_01485) 325659..326372 + 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
KU883_RS01490 (KU883_01490) 326536..326757 + 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KU883_RS01495 (KU883_01495) 326754..327122 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KU883_RS01500 (KU883_01500) 327414..328730 + 1317 WP_022066271.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
KU883_RS01505 (KU883_01505) 328837..329724 + 888 WP_012967120.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
KU883_RS01510 (KU883_01510) 329721..330566 + 846 WP_008806976.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
KU883_RS01515 (KU883_01515) 330568..331638 + 1071 WP_077139459.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 307801..332375 24574


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T210115 WP_002920800.1 NZ_CP079802:326754-327122 [Klebsiella variicola]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T210115 NZ_CP104403:c2364798-2364313 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGGACGTGTAACAGCACCAGAGCCCTTATCCAGCGTCCATCAACTGGCGGAGTTCGTCAGCGGAGAGGCCGTGCTTGA
CGAATGGCTAAAACAAAGAGGTCTGAAAAACCAGGCGCTCGGCGCAGCCCGAACGTTTGTCGTTTGTAAAACGGGCACGA
AGCAGGTCGCGGGATTTTACTCTCTGGCTACCGGCAGCGTTAACCATACGCAAGCGACAGGTAATCTGCGGCGTAACATG
CCGGATCCCATACCCGTGATTATACTTGCCCGTCTGGCTGTGGATGTCTCGTTACGCGGAAACGGGTTGGGAGCCGATTT
ACTCCACGATGCGGTTTTACGCTGTTATCGGGTAGCTGAGAATATCGGGGTGCGGGCGATTATGGTTCATGCACTCACTG
AGGAAGCCAAAGCGTTTTATATCCACCATGGTTTCAAAGCATCGCAGACTCAGGAACGGACACTGTTTCTCAGGCTTCCT
CAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT210115 WP_004174006.1 NZ_CP079802:326536-326757 [Klebsiella variicola]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT210115 NZ_CP104403:c2365052-2364786 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAAATCAGATGTTCAACTCAATCTCAGAGCCAAAGAATCCCAGCGGGCGCTTATCGATGCCGCCGCAGAGATCCTCCA
TAAATCACGCACGGACTTCATTCTGGAGATGGCCTGTCAGGCAGCCGAGAATGTGATCCTCGATCGACGTGTATTCAATT
TTAACGATGAACAGTACGCAGAGTTCATTGACATGCTCGATGCCCCGGTCGCGGACGATCCTGCCATAGAAAAACTGCTG
GCAAGGAAACCGCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References