Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 48403..49025 Replicon plasmid pAJ_082-3
Accession NZ_CP079794
Organism Acinetobacter johnsonii strain AJ_082

Toxin (Protein)


Gene name vapC Uniprot ID A0A7H8SKU4
Locus tag EGT72_RS17585 Protein ID WP_063454755.1
Coordinates 48630..49025 (+) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID R9B9I8
Locus tag EGT72_RS17580 Protein ID WP_000401521.1
Coordinates 48403..48633 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGT72_RS17550 (EGT72_017550) 44099..44434 + 336 WP_035363642.1 hypothetical protein -
EGT72_RS17555 (EGT72_017555) 44484..44663 + 180 WP_016658770.1 hypothetical protein -
EGT72_RS17560 (EGT72_017560) 44660..45046 + 387 WP_004769969.1 type II toxin-antitoxin system death-on-curing family toxin -
EGT72_RS17565 (EGT72_017565) 45048..45599 - 552 WP_016658769.1 macro domain-containing protein -
EGT72_RS17570 (EGT72_017570) 45605..46288 - 684 WP_016658768.1 hypothetical protein -
EGT72_RS17575 (EGT72_017575) 46498..47823 + 1326 Protein_56 ISNCY family transposase -
EGT72_RS17580 (EGT72_017580) 48403..48633 + 231 WP_000401521.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
EGT72_RS17585 (EGT72_017585) 48630..49025 + 396 WP_063454755.1 type II toxin-antitoxin system VapC family toxin Toxin
EGT72_RS17590 (EGT72_017590) 49186..49617 + 432 WP_063454756.1 hypothetical protein -
EGT72_RS17595 (EGT72_017595) 49739..50338 - 600 Protein_60 ISNCY family transposase -
EGT72_RS17605 (EGT72_017605) 51618..52358 - 741 Protein_62 ISNCY-like element ISAba32 family transposase -
EGT72_RS17610 (EGT72_017610) 52566..53518 + 953 Protein_63 IS5 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..155957 155957
- inside IScluster/Tn - - 41753..59330 17577


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-124)

Antitoxin

SpoVT-AbrB domain

(7-37)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14897.23 Da        Isoelectric Point: 7.1633

>T210112 WP_063454755.1 NZ_CP079794:48630-49025 [Acinetobacter johnsonii]
MIYLLDTNICIYVINNKPQQVFERFKQYQLGQLAISSITASELAFGVEKSGSERNKKALNKFLSPLEILPYDEQAIWHYA
KLRQNLQSTGKPIGSLDMLIAAHALALDVVLVTNNTKEFERIDGLKLENWV

Download         Length: 396 bp

>T210112 NZ_CP104403:c1118389-1118171 [Enterobacter hormaechei subsp. steigerwaltii]
ATGTCTGATAAACCATTAACAAAAGTCGATTATTTGATGCGCCTGCGACGCTGCCAGTCAATTGACACCCTTGAACGAGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAGC
TGACCATGAATAAACTGTATGACAAGATCCCCTCTTCGGTGTGGAAATTTGTTCGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8759.04 Da        Isoelectric Point: 4.7708

>AT210112 WP_000401521.1 NZ_CP079794:48403-48633 [Acinetobacter johnsonii]
MEIAKVFQTGRSQAVRLPKAFRFNGTEVAIKSFGRGVLLMPIDNPWDVMLEAINEFEVGFQLERADQGEQLREEFR

Download         Length: 231 bp

>AT210112 NZ_CP104403:c1118791-1118417 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAACGAGCTGATCGAGC
ATATCGCCACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACTAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7H8SKU4

Antitoxin

Source ID Structure
AlphaFold DB A0A2N6V6E6

References