Detailed information of TA system TA210111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 288922..289450
• Replicon: plasmid pAJ_082-2
• Accession: NZ_CP079793
• Organism: Acinetobacter johnsonii strain AJ_082

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EGT72_RS17065
• Protein ID: WP_125280690.1
• Coordinates: 288922..289215 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9B5Q7
• Locus tag: EGT72_RS17070
• Protein ID: WP_000246756.1
• Coordinates: 289205..289450 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGT72_RS17040 (EGT72_017040)	284318..284851	+	534	WP_043041555.1	hypothetical protein	-
EGT72_RS17045 (EGT72_017045)	284890..285531	-	642	WP_024160906.1	N-acetylmuramoyl-L-alanine amidase	-
EGT72_RS17050 (EGT72_017050)	285721..286452	+	732	WP_228740239.1	hypothetical protein	-
EGT72_RS18525	287152..287745	+	594	WP_004758987.1	hypothetical protein	-
EGT72_RS17065 (EGT72_017065)	288922..289215	-	294	WP_125280690.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EGT72_RS17070 (EGT72_017070)	289205..289450	-	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
EGT72_RS17075 (EGT72_017075)	289565..290899	-	1335	WP_125280689.1	ISNCY family transposase	-
EGT72_RS17080 (EGT72_017080)	291134..292483	-	1350	WP_004644748.1	chromate efflux transporter	-
EGT72_RS17085 (EGT72_017085)	292480..293406	-	927	WP_026055779.1	chromate resistance protein	-
EGT72_RS17090 (EGT72_017090)	293597..293947	+	351	WP_004644745.1	DUF3147 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / msr(E) / mph(E) / blaNDM-1 / ARR-3 / qacE / sul1 / aph(3')-VIa	-	1..340235	340235
	-	inside	IScluster/Tn	-	-	287755..295513	7758

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-71)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11304.58 Da
• Isoelectric Point: 10.4753

>T210111 WP_125280690.1 NZ_CP079793:c289215-288922 [Acinetobacter johnsonii]
MTYKLLRHKDFIAEWEKLPVAIRDQFKKKLAKVIEQPHIPKNMLRGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Nucleotide

• Length: 294 bp

>T210111 NZ_CP104403:651983-652270 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCCTATAGAGTATGAGTGGGATAGCAATAAGGCGAAAAGCAATCTGCAAAAACACGCCATTCGTTTTGAAGATGCAGT
TCTGGTTTTTGACGATCCCTGCCATTTGTCTGTTCAGGATCGTTATGAAAACGGGGAGTTTCGCTGGCAAACCATTGGTC
TTGTACAGGGCGTTATTGTCATTCTGGTCGCGCACACGGTACGTTTTGAAAGCGGGGATGAAATCATACGTATCATCAGT
GCGCGTAAAGCGGATCGTAAAGAGAGGAGGCGTTATGAGCATCGTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9235.61 Da
• Isoelectric Point: 4.3142

>AT210111 WP_000246756.1 NZ_CP079793:c289450-289205 [Acinetobacter johnsonii]
MATVNFRVDEALKEKSYSILKEQGIAPTDFFTSILEYVATTGKLPVKKALLSEEDEELLALVRKRINDPKEMFEEVTLDD
L

Nucleotide

• Length: 246 bp

>AT210111 NZ_CP104403:652257-652559 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAGCATCGTTAAACATAAACGCGGTAGTGCACCGGAAGTGAGCGCCCGCCGGGAGGCTGAACTCAAAGCGCTGGCGGA
TAAACCCGACAGGGAAATTGATTACAGCGATATACCGGCCACGGAGGATGATCGCTGGGCCGGTGCCGTGCGGGGAAAAT
TCTATCGACCGCTCAAAACTCAGGCATCAGTACGCATTGACGCCGACGTGATGGAGTGGCTTAAACGGCCAGGGAAAGGT
TACCAGACGCGGCTTAATGCCATTTTGCGCGAGGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: N9B5Q7