Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | BrnTA/BrnT-BrnA |
| Location | 168084..168672 | Replicon | plasmid pAJ_082-2 |
| Accession | NZ_CP079793 | ||
| Organism | Acinetobacter johnsonii strain AJ_082 | ||
Toxin (Protein)
| Gene name | brnT | Uniprot ID | N8SM64 |
| Locus tag | EGT72_RS16360 | Protein ID | WP_000438825.1 |
| Coordinates | 168084..168371 (+) | Length | 96 a.a. |
Antitoxin (Protein)
| Gene name | brnA | Uniprot ID | D3JXQ4 |
| Locus tag | EGT72_RS16365 | Protein ID | WP_004728120.1 |
| Coordinates | 168358..168672 (+) | Length | 105 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EGT72_RS16340 (EGT72_016340) | 164264..165456 | + | 1193 | WP_085955245.1 | IS3-like element ISKpn18 family transposase | - |
| EGT72_RS16345 (EGT72_016345) | 165575..166189 | + | 615 | WP_125281634.1 | recombinase family protein | - |
| EGT72_RS16350 (EGT72_016350) | 166255..167058 | + | 804 | WP_125281635.1 | aminoglycoside O-phosphotransferase APH(3'')-Ib | - |
| EGT72_RS16355 (EGT72_016355) | 167058..167894 | + | 837 | WP_125281636.1 | APH(6)-I family aminoglycoside O-phosphotransferase | - |
| EGT72_RS16360 (EGT72_016360) | 168084..168371 | + | 288 | WP_000438825.1 | BrnT family toxin | Toxin |
| EGT72_RS16365 (EGT72_016365) | 168358..168672 | + | 315 | WP_004728120.1 | BrnA antitoxin family protein | Antitoxin |
| EGT72_RS16370 (EGT72_016370) | 168761..169291 | - | 531 | WP_000172759.1 | hypothetical protein | - |
| EGT72_RS16375 (EGT72_016375) | 169440..169742 | + | 303 | WP_000286964.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| EGT72_RS16380 (EGT72_016380) | 169743..170024 | + | 282 | WP_000985609.1 | putative addiction module antidote protein | - |
| EGT72_RS16385 (EGT72_016385) | 170104..170523 | - | 420 | WP_001180106.1 | SMI1/KNR4 family protein | - |
| EGT72_RS16390 (EGT72_016390) | 170663..170983 | + | 321 | WP_219356867.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| EGT72_RS16395 (EGT72_016395) | 170976..171248 | + | 273 | WP_000369781.1 | NadS family protein | - |
| EGT72_RS16400 (EGT72_016400) | 171741..173216 | + | 1476 | WP_000052512.1 | ABC-F type ribosomal protection protein Msr(E) | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | sul2 / aph(3'')-Ib / aph(6)-Id / msr(E) / mph(E) / blaNDM-1 / ARR-3 / qacE / sul1 / aph(3')-VIa | - | 1..340235 | 340235 | |
| - | inside | IScluster/Tn | aph(3'')-Ib / aph(6)-Id / msr(E) / mph(E) | - | 159969..174156 | 14187 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 96 a.a. Molecular weight: 11304.70 Da Isoelectric Point: 5.6205
>T210108 WP_000438825.1 NZ_CP079793:168084-168371 [Acinetobacter johnsonii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTDGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG
Download Length: 288 bp
>T210108 NZ_CP104403:c298733-298446 [Enterobacter hormaechei subsp. steigerwaltii]
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTGAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA
ATGCATAAATTAACACCCTACGATCCAGCCAACGCACTGGTGGATGACGAGGAAATCGCTGTGTTTATGGCTGATGCATT
AGAGACGGGTGACTCAGCGTACATTGCTGAAGCGCTGGGCGTCATCGCCCGAGCGAAAGGGATGTCGACCATTTCCCAGC
AAACGGGCCTGTCACGAGAACAACTGTATCGATCATTCAGTGATAAGGGGAACCCAACGCTCAAAACCACGCTGGCGGTC
ATGAAAGCATTGGGCCTTGGGTTAACAATCAAACCCTCTGGGGATTAA
Antitoxin
Download Length: 105 a.a. Molecular weight: 12200.86 Da Isoelectric Point: 8.4628
>AT210108 WP_004728120.1 NZ_CP079793:168358-168672 [Acinetobacter johnsonii]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSSAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ
Download Length: 315 bp
>AT210108 NZ_CP104403:c299042-298740 [Enterobacter hormaechei subsp. steigerwaltii]
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA
ATGAAGGAGATCGTTCAGACAGAATCCTTCCGACGCTGGGAGCAAAATTTAAAGGACAGGCGGGCAAAGACGATTATCGC
TTCCCGCCTCTTTAGGCTGGCAAATGGCTTAGCGGGCGACATTAGACCCGTGGGGGAAGGTATCAGTGAACTGAGGATCC
ACTTTGGCCCGGGCTACAGAGTCTATTTTAAGGACCAGGGCAATTGCATCATCGTGCTGTTATGTGGTGGTGACAAAAGC
AGCCAGGCCAGAGACATACTTATGGCAAAAATGCTGAGCAATGTATCCCAATGGCAGGAGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TVQ4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0A8TRI9 |