Detailed information of TA system TA210104

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 49310..50220
• Replicon: plasmid pKPT698-tmexCD
• Accession: NZ_CP079784
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain T698-1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: KYY07_RS27595
• Protein ID: WP_040168660.1
• Coordinates: 49750..50220 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A2J4RLY9
• Locus tag: KYY07_RS27590
• Protein ID: WP_004181895.1
• Coordinates: 49310..49753 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYY07_RS27575 (KYY07_27500)	45943..47430	+	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
KYY07_RS27580 (KYY07_27505)	47516..47938	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
KYY07_RS27585 (KYY07_27510)	47938..49209	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
KYY07_RS27590 (KYY07_27515)	49310..49753	+	444	WP_004181895.1	DUF2384 domain-containing protein	Antitoxin
KYY07_RS27595 (KYY07_27520)	49750..50220	+	471	WP_040168660.1	RES family NAD+ phosphorylase	Toxin
KYY07_RS27600 (KYY07_27525)	50330..50590	-	261	WP_004026352.1	hypothetical protein	-
KYY07_RS27605 (KYY07_27530)	51161..52340	-	1180	Protein_65	IS481 family transposase	-
KYY07_RS27610 (KYY07_27535)	52412..52570	+	159	WP_004181898.1	type I toxin-antitoxin system Hok family toxin	-
KYY07_RS27615 (KYY07_27540)	53173..54147	+	975	WP_040222313.1	hypothetical protein	-
KYY07_RS27620 (KYY07_27545)	54345..54722	-	378	WP_004181901.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(6)-Id / aph(3'')-Ib / aph(3')-Ia / mph(E) / msr(E) / armA / sul1 / blaDHA-1 / qnrB4	-	1..291624	291624

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17531.83 Da
• Isoelectric Point: 4.6155

>T210104 WP_040168660.1 NZ_CP079784:49750-50220 [Klebsiella pneumoniae subsp. pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSLVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T210104 NZ_CP104401:c3892816-3892406 [Enterobacter hormaechei subsp. steigerwaltii]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCCTTACTGCTCCACGGGCTGGTGGCGGC
ACTGGTGTTATTGGTGCCGTGGCCGCTTAGCTATACCCCTCTGTGGTTGCTATTGCTGTCGTTTGTGGTGTTTGACAGCG
TGCGCAGCCAGCGTCGAATCAACGCCCGTCAGGGGGAAATTAAGCTGCTGATGGATTCTCGCCTGCGCTGGCAGGGAAAA
GAGTGGGAGATGATGGGGACGCCGTGGATGCTTAATACCGGGATGATGCTACGGCTGCGTCGGGTCGAGGATAACCGTCG
CCAGCACCTGTGGCTGGCCGCAGACAGTATGGATCCCGCGGAGTGGCGAGATCTGCGCCGGTTGATCGTGCAACAGCCGA
CGCAGGAGTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16536.87 Da
• Isoelectric Point: 10.3013

>AT210104 WP_004181895.1 NZ_CP079784:49310-49753 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT210104 NZ_CP104401:c3893063-3892797 [Enterobacter hormaechei subsp. steigerwaltii]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATCTCCATCATGCCTTT
CTTCGAGTATGAGTACGACAGCTTAAGCGATGACGATAAGCGCCTGTTTGTCCGTCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAATCACGGCAAGCCCGCCGACACCGAGTTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CAGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J4RLY9