Detailed information of TA system TA210103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 32286..32811
• Replicon: plasmid pKPT698-mcr
• Accession: NZ_CP079783
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain T698-1

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: KYY07_RS26935
• Protein ID: WP_004197633.1
• Coordinates: 32506..32811 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: KYY07_RS26930
• Protein ID: WP_004197642.1
• Coordinates: 32286..32504 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYY07_RS26910 (KYY07_26845)	27551..28531	+	981	WP_072311004.1	IS5-like element ISKpn26 family transposase	-
KYY07_RS26915 (KYY07_26850)	28973..29764	-	792	WP_221928726.1	ribbon-helix-helix domain-containing protein	-
KYY07_RS26920 (KYY07_26855)	29947..30975	-	1029	WP_032445668.1	Abi family protein	-
KYY07_RS26925 (KYY07_26860)	31136..31717	-	582	WP_072310991.1	hypothetical protein	-
KYY07_RS26930 (KYY07_26865)	32286..32504	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
KYY07_RS26935 (KYY07_26870)	32506..32811	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
KYY07_RS26940 (KYY07_26875)	32980..33375	+	396	WP_164530166.1	hypothetical protein	-
KYY07_RS26945 (KYY07_26880)	33402..33725	+	324	WP_004197641.1	hypothetical protein	-
KYY07_RS26950 (KYY07_26885)	33722..34738	+	1017	WP_164530167.1	hypothetical protein	-
KYY07_RS26955 (KYY07_26890)	34936..35721	+	786	WP_046664219.1	site-specific integrase	-
KYY07_RS26960 (KYY07_26895)	36170..36925	+	756	WP_001568031.1	replication initiation protein RepE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-15 / blaTEM-1B / mcr-8	-	1..90695	90695

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T210103 WP_004197633.1 NZ_CP079783:32506-32811 [Klebsiella pneumoniae subsp. pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T210103 NZ_CP104401:3664500-3664799 [Enterobacter hormaechei subsp. steigerwaltii]
ATGATTAATCATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTCTACGGGAGATCGAGCAATGTTATCCCTTTGAATCT
GGAAACAGCGCTTGCAAGAAAGCTCGACATCATCAATGCGGCCATGTCGCACCTGGATTTACGATCGCCACCGGGCAATA
TGTATGAAGCATTGAGTCCTCCGTTGAAGGGATATTCCTCTATCCGGGTAAACAGACAATACAGGCTTGTATTTCGCTGG
TCAGAGGGAAAAGCAGACGATCTCTATCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT210103 WP_004197642.1 NZ_CP079783:32286-32504 [Klebsiella pneumoniae subsp. pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT210103 NZ_CP104401:3664810-3665175 [Enterobacter hormaechei subsp. steigerwaltii]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGAGGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAACGATCTGGCAGAGATGCTAAATGTACACCGCAATACCGTAAGCGCATTGGTCAATAACAATCGCAAACTTA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTAATACCACTATTGAATTTTGGCTGAACTTACAGCTAAACGTTGAT
ATCTGGGAAGCGCAATCTAACTCCCGCACGCAGGAGGAGTTAAGCCGGATAAAAACCGTTGCGGAAGTCATGGCGAAGCG
AAAATCCGGCAAGCCAGACGTTACCCTGCACGGGAACAGCGCATAA

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5