Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 23906..24549 Replicon plasmid pKPT698-tetA
Accession NZ_CP079782
Organism Klebsiella pneumoniae subsp. pneumoniae strain T698-1

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag KYY07_RS26110 Protein ID WP_000754566.1
Coordinates 24133..24549 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag KYY07_RS26105 Protein ID WP_001261276.1
Coordinates 23906..24136 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYY07_RS26065 (KYY07_26010) 19443..19556 + 114 WP_014343462.1 hypothetical protein -
KYY07_RS26070 (KYY07_26015) 19685..19942 + 258 WP_241510519.1 hypothetical protein -
KYY07_RS26075 (KYY07_26020) 20000..20779 - 780 WP_023287113.1 site-specific integrase -
KYY07_RS26080 (KYY07_26025) 20977..21993 - 1017 WP_009309921.1 hypothetical protein -
KYY07_RS26085 (KYY07_26030) 22027..22362 - 336 WP_009309920.1 hypothetical protein -
KYY07_RS26090 (KYY07_26035) 22412..22723 - 312 WP_009309919.1 hypothetical protein -
KYY07_RS26095 (KYY07_26040) 22781..23086 - 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB -
KYY07_RS26100 (KYY07_26045) 23088..23306 - 219 WP_004197642.1 type II toxin-antitoxin system antitoxin CcdA -
KYY07_RS26105 (KYY07_26050) 23906..24136 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KYY07_RS26110 (KYY07_26055) 24133..24549 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
KYY07_RS26115 (KYY07_26060) 24590..25468 - 879 WP_009309916.1 restriction endonuclease -
KYY07_RS26120 (KYY07_26065) 26130..27407 + 1278 WP_009309915.1 HlyD family secretion protein -
KYY07_RS26125 (KYY07_26070) 27397..29505 + 2109 WP_032427226.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / sul1 / catA2 / tet(A) mrkA / mrkB / mrkC / mrkD / mrkF / mrkJ 1..138834 138834
- flank IS/Tn - - 18360..19340 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T210101 WP_000754566.1 NZ_CP079782:24133-24549 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T210101 NZ_CP104401:c2804020-2803917 [Enterobacter hormaechei subsp. steigerwaltii]
GGCAAGGCGATTGAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT210101 WP_001261276.1 NZ_CP079782:23906-24136 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT210101 NZ_CP104401:2803904-2804113 [Enterobacter hormaechei subsp. steigerwaltii]
TTTTCAAGCAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGC
TAAAAGTTGGCATTAATGCAGGCTCAATCGCCTTGCCCTTTAAGAATAGATGACGACGTCAGGTTTTCCAGTCCACAGTA
AAAGTGGTCTGAAAAAAAGCGTCAGAACATCACTAAATGTGAAAAACCGC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References