Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4113297..4113915 Replicon chromosome
Accession NZ_CP079780
Organism Escherichia coli strain BL12

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KYX59_RS22055 Protein ID WP_001291435.1
Coordinates 4113297..4113515 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KYX59_RS22060 Protein ID WP_000344800.1
Coordinates 4113541..4113915 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX59_RS22020 (4108586) 4108586..4109158 + 573 WP_000779831.1 YbaY family lipoprotein -
KYX59_RS22025 (4109189) 4109189..4109500 - 312 WP_000409911.1 MGMT family protein -
KYX59_RS22035 (4109879) 4109879..4110232 + 354 WP_000878140.1 DUF1428 domain-containing protein -
KYX59_RS22040 (4110274) 4110274..4111824 - 1551 WP_023281027.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KYX59_RS22045 (4111988) 4111988..4112458 - 471 WP_000136192.1 YlaC family protein -
KYX59_RS22050 (4112574) 4112574..4113125 - 552 WP_000102564.1 maltose O-acetyltransferase -
KYX59_RS22055 (4113297) 4113297..4113515 - 219 WP_001291435.1 HHA domain-containing protein Toxin
KYX59_RS22060 (4113541) 4113541..4113915 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KYX59_RS22065 (4114461) 4114461..4117610 - 3150 WP_218077447.1 efflux RND transporter permease AcrB -
KYX59_RS22070 (4117633) 4117633..4118826 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T210081 WP_001291435.1 NZ_CP079780:c4113515-4113297 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T210081 NZ_CP104386:718152-718553 [Cupriavidus gilardii]
ATGGCGTTGTACCTGCTTGACACGAATATCGTCTCGGCGGCCCTTCGGAATACCCGCGGCGCCTGTGCCGCGCGGATCGG
AATGACGGACGCCGGGGCGCTGTGCACGTCCATTGTCGTCGCGGCCGAACTGCGCTTCGGCGTTGCGAAGAAGGGTTCTG
CCGAGTTGGCACGCCGGGTGGAACATGCCTTGAGCACGCTTCGCGTGTTGCCGCTCGATGGCGATGCCGATCGGCACTAT
GGCCGGCTGCGCACCGAGCTGGAGCGCCATGGGAAACCGATCGGCGCCAACGATATGCTGATCGCCGCGCATGCCCTGGC
GATTGACGCTGTACTCGTGACGGACAACGTGTCCGAGTTTTCGCGCGTGCCGGGTTTGCGCTACGAAAACTGGCTGACTT
GA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT210081 WP_000344800.1 NZ_CP079780:c4113915-4113541 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT210081 NZ_CP104386:717898-718152 [Cupriavidus gilardii]
ATGACAACGAAGACGGATGCCACGTCGGAAGCCAGGCATGTGCGGCTTTTCCGCAATGGCCGCAATCAGGCGGTTCGGAT
ACCGCGCGAGTTCGAGTTACCTGGAGAGGAGGCGATCATGCGTAAAGAAGGCGACAAGCTGATCATCGAACCGATTCCTG
CGACGTCGTTCCGGGCGCTTCTCGCGCAATGGGAGCCGCTGGACGAGACGTGGCCGGACATCGATGACCGGCCTCCCGAG
CCGGTGGATCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References