Detailed information of TA system TA210077

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3397083..3397918
• Replicon: chromosome
• Accession: NZ_CP079780
• Organism: Escherichia coli strain BL12

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: KYX59_RS18620
• Protein ID: WP_000854821.1
• Coordinates: 3397083..3397460 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KYX59_RS18625
• Protein ID: WP_032216526.1
• Coordinates: 3397550..3397918 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX59_RS18595 (3392673)	3392673..3394211	-	1539	WP_001187177.1	lysine decarboxylation/transport transcriptional activator CadC	-
KYX59_RS18600 (3394524)	3394524..3395221	+	698	WP_095033700.1	IS1-like element IS1B family transposase	-
KYX59_RS18605 (3395737)	3395737..3396579	-	843	WP_001529559.1	DUF4942 domain-containing protein	-
KYX59_RS18610 (3396664)	3396664..3396861	-	198	WP_023563519.1	DUF957 domain-containing protein	-
KYX59_RS18615 (3396937)	3396937..3397086	-	150	Protein_3247	DUF5983 family protein	-
KYX59_RS18620 (3397083)	3397083..3397460	-	378	WP_000854821.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KYX59_RS18625 (3397550)	3397550..3397918	-	369	WP_032216526.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KYX59_RS18630 (3397937)	3397937..3398581	-	645	Protein_3250	antitoxin of toxin-antitoxin stability system	-
KYX59_RS18635 (3398600)	3398600..3398821	-	222	WP_001601167.1	DUF987 domain-containing protein	-
KYX59_RS18640 (3398890)	3398890..3399366	-	477	WP_001186724.1	RadC family protein	-
KYX59_RS18645 (3399382)	3399382..3399867	-	486	WP_165467258.1	antirestriction protein	-
KYX59_RS18650 (3399959)	3399959..3400777	-	819	WP_053902307.1	DUF932 domain-containing protein	-
KYX59_RS18655 (3400876)	3400876..3401109	-	234	WP_001278282.1	DUF905 family protein	-
KYX59_RS18660 (3401115)	3401115..3401792	-	678	WP_259481943.1	hypothetical protein	-
KYX59_RS18665 (3401911)	3401911..3402795	-	885	WP_032216515.1	50S ribosome-binding GTPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3394844..3395221	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T210077 WP_000854821.1 NZ_CP079780:c3397460-3397083 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T210077 NZ_CP104384:3804435-3804713 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13810.69 Da
• Isoelectric Point: 6.2066

>AT210077 WP_032216526.1 NZ_CP079780:c3397918-3397550 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLLCTVTPCFGARLVQEGNRLHYLVDRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT210077 NZ_CP104384:3804172-3804432 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: A0A7W4PV54