Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2086092..2086746 Replicon chromosome
Accession NZ_CP079780
Organism Escherichia coli strain BL12

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KYX59_RS12350 Protein ID WP_000244777.1
Coordinates 2086092..2086499 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KYX59_RS12355 Protein ID WP_000354046.1
Coordinates 2086480..2086746 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX59_RS12330 (2082049) 2082049..2083782 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
KYX59_RS12335 (2083788) 2083788..2084498 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KYX59_RS12340 (2084523) 2084523..2085419 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KYX59_RS12345 (2085531) 2085531..2086052 + 522 WP_001055874.1 flavodoxin FldB -
KYX59_RS12350 (2086092) 2086092..2086499 - 408 WP_000244777.1 protein YgfX Toxin
KYX59_RS12355 (2086480) 2086480..2086746 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KYX59_RS12360 (2086989) 2086989..2087969 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KYX59_RS12365 (2088165) 2088165..2088824 - 660 WP_000250274.1 hemolysin III family protein -
KYX59_RS12370 (2088988) 2088988..2089299 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KYX59_RS12375 (2089344) 2089344..2090777 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
KYX59_RS12380 (2090834) 2090834..2091577 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T210072 WP_000244777.1 NZ_CP079780:c2086499-2086092 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T210072 NZ_CP104384:2673542-2673649 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT210072 WP_000354046.1 NZ_CP079780:c2086746-2086480 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT210072 NZ_CP104384:c2673495-2673429 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References