Detailed information of TA system TA210054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/MqsA(antitoxin)
• Location: 13152..13617
• Replicon: plasmid pBL12EC-4
• Accession: NZ_CP079779
• Organism: Escherichia coli strain BL12

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: U9ZUY2
• Locus tag: KYX59_RS01820
• Protein ID: WP_001230707.1
• Coordinates: 13366..13617 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: M7CCF8
• Locus tag: KYX59_RS01815
• Protein ID: WP_000801440.1
• Coordinates: 13152..13379 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX59_RS01775 (KYX59_01725)	8382..8597	-	216	WP_000760375.1	EexN family lipoprotein	-
KYX59_RS01780 (KYX59_01730)	8594..8887	-	294	WP_000134733.1	sigma factor-like helix-turn-helix DNA-binding protein	-
KYX59_RS01785 (KYX59_01735)	8964..10193	-	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
KYX59_RS01790 (KYX59_01740)	10197..10700	-	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
KYX59_RS01795 (KYX59_01745)	11061..11408	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	-
KYX59_RS01800 (KYX59_01750)	11405..11653	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	-
KYX59_RS01805 (KYX59_01755)	11958..12308	-	351	WP_000854259.1	hypothetical protein	-
KYX59_RS01810 (KYX59_01760)	12399..12698	-	300	WP_000650309.1	hypothetical protein	-
KYX59_RS01815 (KYX59_01765)	13152..13379	-	228	WP_000801440.1	hypothetical protein	Antitoxin
KYX59_RS01820 (KYX59_01770)	13366..13617	-	252	WP_001230707.1	hypothetical protein	Toxin
KYX59_RS01825 (KYX59_01775)	13614..14156	-	543	WP_001083906.1	hypothetical protein	-
KYX59_RS01830 (KYX59_01780)	14261..14776	-	516	WP_001025395.1	J domain-containing protein	-
KYX59_RS01835 (KYX59_01785)	16191..17057	+	867	WP_001353743.1	replication initiation protein	-
KYX59_RS01840 (KYX59_01790)	17070..17474	+	405	WP_012421203.1	hypothetical protein	-
KYX59_RS01845 (KYX59_01795)	17609..18136	+	528	WP_000577043.1	DUF2726 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..37819	37819

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-69)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9751.13 Da
• Isoelectric Point: 8.9187

>T210054 WP_001230707.1 NZ_CP079779:c13617-13366 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Nucleotide

• Length: 252 bp

>T210054 NZ_CP104373:c4821644-4821360 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8659.98 Da
• Isoelectric Point: 8.5105

>AT210054 WP_000801440.1 NZ_CP079779:c13379-13152 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Nucleotide

• Length: 228 bp

>AT210054 NZ_CP104373:c4821876-4821634 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAACAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: U9ZUY2

Antitoxin

• AlphaFold DB: A0A5Y6U8W9