Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 111363..111889 Replicon plasmid pBL12EC-2
Accession NZ_CP079777
Organism Escherichia coli strain BL12

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag KYX59_RS01230 Protein ID WP_000323025.1
Coordinates 111363..111650 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag KYX59_RS01235 Protein ID WP_000534858.1
Coordinates 111650..111889 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KYX59_RS01200 106960..107040 - 81 Protein_118 hypothetical protein -
KYX59_RS01205 (KYX59_01185) 107028..107453 + 426 Protein_119 IS3 family transposase -
KYX59_RS01210 (KYX59_01190) 108126..109634 + 1509 WP_139245108.1 group II intron reverse transcriptase/maturase -
KYX59_RS01215 (KYX59_01195) 109715..110204 + 490 Protein_121 IS3 family transposase -
KYX59_RS01220 (KYX59_01200) 110210..110419 - 210 WP_072662162.1 hypothetical protein -
KYX59_RS01225 (KYX59_01205) 110856..111080 - 225 Protein_123 hypothetical protein -
KYX59_RS01230 (KYX59_01210) 111363..111650 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
KYX59_RS01235 (KYX59_01215) 111650..111889 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
KYX59_RS01240 111914..112024 + 111 Protein_126 hypothetical protein -
KYX59_RS01245 (KYX59_01220) 112231..112380 + 150 WP_000550473.1 hypothetical protein -
KYX59_RS01250 (KYX59_01225) 112424..113497 + 1074 WP_000557466.1 hypothetical protein -
KYX59_RS01255 (KYX59_01230) 113574..114278 + 705 WP_001067852.1 IS6-like element IS26 family transposase -
KYX59_RS01260 (KYX59_01235) 114330..114668 + 339 Protein_130 recombinase family protein -
KYX59_RS01265 (KYX59_01240) 114700..115404 + 705 Protein_131 IS110 family transposase -
KYX59_RS01270 (KYX59_01245) 115743..116102 + 360 WP_171960221.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / qnrS1 / dfrA14 / blaTEM-1C / floR - 1..119769 119769


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T210049 WP_000323025.1 NZ_CP079777:c111650-111363 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T210049 NZ_CP104373:2063153-2063255 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT210049 WP_000534858.1 NZ_CP079777:c111889-111650 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT210049 NZ_CP104373:c2063262-2063117 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References