Detailed information of TA system TA210046

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 12354..12952
• Replicon: plasmid pBL12EC-1
• Accession: NZ_CP079776
• Organism: Escherichia coli strain BL12

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: KYX59_RS00080
• Protein ID: WP_053291281.1
• Coordinates: 12354..12731 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: KYX59_RS00085
• Protein ID: WP_001603498.1
• Coordinates: 12731..12952 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KYX59_RS00055 (KYX59_00055)	7366..7719	+	354	WP_161953766.1	hypothetical protein	-
KYX59_RS00060 (KYX59_00060)	7772..8539	-	768	WP_001711193.1	hypothetical protein	-
KYX59_RS00065 (KYX59_00065)	8822..9547	+	726	WP_033545990.1	hypothetical protein	-
KYX59_RS00070 (KYX59_00070)	9608..10948	+	1341	WP_259483151.1	DnaB-like helicase C-terminal domain-containing protein	-
KYX59_RS00075 (KYX59_00075)	11036..12220	+	1185	WP_023135654.1	hypothetical protein	-
KYX59_RS00080 (KYX59_00080)	12354..12731	-	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KYX59_RS00085 (KYX59_00085)	12731..12952	-	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KYX59_RS00090 (KYX59_00090)	13137..14147	-	1011	WP_259483147.1	fimbrial protein	-
KYX59_RS00095 (KYX59_00095)	14158..16644	-	2487	WP_259483145.1	fimbrial biogenesis outer membrane usher protein	-
KYX59_RS00100 (KYX59_00100)	16662..17342	-	681	WP_032187672.1	fimbrial assembly chaperone	-
KYX59_RS00105 (KYX59_00105)	17398..17946	-	549	WP_166498870.1	fimbrial protein YehD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..106228	106228

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T210046 WP_053291281.1 NZ_CP079776:c12731-12354 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T210046 NZ_CP104373:328836-329204 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT210046 WP_001603498.1 NZ_CP079776:c12952-12731 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT210046 NZ_CP104373:328618-328839 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: L4J1C0